Circadian rhythmicity of interferon-gamma production in antigen-stimulated whole blood

N. Petrovsky; P. McNair; L. C. Harrison

Circadian rhythmicity of interferon-gamma production in antigen-stimulated whole blood

N. Petrovsky, P. McNair, L. C. Harrison

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-γ. With this assay we have found a large circadian variation in tetanus-(acrophase 00⁰⁰ p < 0.001) and PPD- (acrophase 00⁰⁸ p < 0.001) stimulated IFN-γ production. IFN-γ production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-γ production may be secondary to circadian variation in plasma cortisol levels (acrophase 11⁰⁶). The demonstration of circadian rhythmicity in antigen-stimulated IFN-γ production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.

Original language	English
Pages (from-to)	293-300
Number of pages	8
Journal	Chronobiologia
Volume	21
Issue number	3-4
Publication status	Published - 1 Dec 1994
Externally published	Yes

Keywords

blood
circadian
cortisol
immunity
interferon-gamma

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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abstract = "Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-γ. With this assay we have found a large circadian variation in tetanus-(acrophase 0000 p < 0.001) and PPD- (acrophase 0008 p < 0.001) stimulated IFN-γ production. IFN-γ production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-γ production may be secondary to circadian variation in plasma cortisol levels (acrophase 1106). The demonstration of circadian rhythmicity in antigen-stimulated IFN-γ production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.",

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AU - Petrovsky, N.

AU - McNair, P.

AU - Harrison, L. C.

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N2 - Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-γ. With this assay we have found a large circadian variation in tetanus-(acrophase 0000 p < 0.001) and PPD- (acrophase 0008 p < 0.001) stimulated IFN-γ production. IFN-γ production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-γ production may be secondary to circadian variation in plasma cortisol levels (acrophase 1106). The demonstration of circadian rhythmicity in antigen-stimulated IFN-γ production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.

AB - Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-γ. With this assay we have found a large circadian variation in tetanus-(acrophase 0000 p < 0.001) and PPD- (acrophase 0008 p < 0.001) stimulated IFN-γ production. IFN-γ production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-γ production may be secondary to circadian variation in plasma cortisol levels (acrophase 1106). The demonstration of circadian rhythmicity in antigen-stimulated IFN-γ production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.

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KW - cortisol

KW - immunity

KW - interferon-gamma

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ER -

Circadian rhythmicity of interferon-gamma production in antigen-stimulated whole blood

Abstract

Keywords

UN SDGs

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