TY - JOUR
T1 - Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis
AU - Santamaria-Martos, Fernando
AU - Benítez, Iván
AU - Ortega, Francisco
AU - Zapater, Andrea
AU - Giron, Cristina
AU - Pinilla, Lucía
AU - Pascual, Lydia
AU - Cortijo, Anunciación
AU - Dalmases, Mireia
AU - Fernandez-Real, Jose M.
AU - Barbé, Ferran
AU - Sánchez-de-la-Torre, Manuel
PY - 2019/9/17
Y1 - 2019/9/17
N2 - Evaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of continuous positive airway pressure (CPAP) treatment on the miRNA profile. This observational, longitudinal study included 230 subjects referred to the Sleep Unit due to suspected OSA. Expression profiling of 188 miRNAs in plasma was performed in 27 subjects by TaqMan-Low-Density-Array. OSA-related miRNAs were selected for validation by RT-qPCR in 203 patients. Prediction models were built to discriminate between non-OSA and OSA: 1) NoSAS-score, 2) differentially expressed miRNAs, and 3) combination of NoSAS-score plus miRNAs. The differentially expressed miRNAs were measured after 6 months of follow-up. From the 14 miRNAs selected for validation, 6 were confirmed to be differentially expressed. The areas under the curve were 0.73 for the NoSAS-score, 0.81 for the miRNAs and 0.86 for the combination. After 6 months of CPAP treatment, miRNA levels in the OSA group seem to approximate to non-OSA levels. A cluster of miRNAs was identified to differentiate between non-OSA and OSA patients. CPAP treatment was associated with changes in the circulating miRNA profile.
AB - Evaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of continuous positive airway pressure (CPAP) treatment on the miRNA profile. This observational, longitudinal study included 230 subjects referred to the Sleep Unit due to suspected OSA. Expression profiling of 188 miRNAs in plasma was performed in 27 subjects by TaqMan-Low-Density-Array. OSA-related miRNAs were selected for validation by RT-qPCR in 203 patients. Prediction models were built to discriminate between non-OSA and OSA: 1) NoSAS-score, 2) differentially expressed miRNAs, and 3) combination of NoSAS-score plus miRNAs. The differentially expressed miRNAs were measured after 6 months of follow-up. From the 14 miRNAs selected for validation, 6 were confirmed to be differentially expressed. The areas under the curve were 0.73 for the NoSAS-score, 0.81 for the miRNAs and 0.86 for the combination. After 6 months of CPAP treatment, miRNA levels in the OSA group seem to approximate to non-OSA levels. A cluster of miRNAs was identified to differentiate between non-OSA and OSA patients. CPAP treatment was associated with changes in the circulating miRNA profile.
KW - MicroRNA profile
KW - Obstructive sleep apnea
KW - Continuous positive airway pressure (CPAP)
KW - Longitudinal study
UR - http://www.scopus.com/inward/record.url?scp=85072297685&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-49940-1
DO - 10.1038/s41598-019-49940-1
M3 - Article
C2 - 31530881
AN - SCOPUS:85072297685
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
M1 - 13456
ER -