Cloning and functional characterization of a peroxiredoxin 4 from yellowtail kingfish (Seriola lalandi)

Hsi-Mei Loo, Kathryn Schuller

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    Peroxiredoxins (Prxs) are antioxidant enzymes with a conserved Cys residue at the active site which undergoes peroxide-dependent oxidation followed by thiol-dependent reduction during each catalytic cycle. In mammals, Prx 4 proteins have an N-terminal signal peptide which targets them for secretion from the cell. This has not been investigated in fish. Thus, here we describe the cloning of a Prx 4 cDNA from yellowtail kingfish (YTK, Seriola lalandi) and the elucidation of the signal peptide cleavage site. The YTK Prx 4 cDNA included a 792bp open reading frame (ORF) encoding a predicted protein of 264 amino acids (referred to as Prx264). SignalP software and a multiple sequence alignment predicted signal peptide cleavage sites after residues 33 and 67 giving rise to Prx231 and Prx197, respectively. All three proteins were expressed in E. coli and assayed for thioredoxin-dependent peroxidase activity. Prx231 and Prx197 both exhibited activity whereas Prx264 did not. Thus, either potential cleavage site may be physiologically relevant. Both active forms of the enzyme displayed a single-displacement reaction mechanism and typical saturable Michaelis-Menten type kinetics. This is unusual for Prx enzymes. In addition, they both displayed a clear preference for H2O2 over cumene hydroperoxide or t-butyl hydroperoxide.

    Original languageEnglish
    Pages (from-to)244-253
    Number of pages10
    JournalComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
    Issue number4
    Publication statusPublished - Aug 2010


    • Peroxiredoxins
    • Prx 4 cDNA
    • Seriola lalandi


    Dive into the research topics of 'Cloning and functional characterization of a peroxiredoxin 4 from yellowtail kingfish (Seriola lalandi)'. Together they form a unique fingerprint.

    Cite this