Cloning of lymphocytes from whole blood by limiting dilution

Kevin J. Trainor, Alexander A. Morley

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.

    Original languageEnglish
    Pages (from-to)369-372
    Number of pages4
    JournalJournal of Immunological Methods
    Volume65
    Issue number3
    DOIs
    Publication statusPublished - 30 Dec 1983

    Keywords

    • cloning frequency
    • limiting dilution cloning
    • lymphocyte cloning

    Fingerprint Dive into the research topics of 'Cloning of lymphocytes from whole blood by limiting dilution'. Together they form a unique fingerprint.

  • Cite this