TY - JOUR
T1 - Cloning of lymphocytes from whole blood by limiting dilution
AU - Trainor, Kevin J.
AU - Morley, Alexander A.
PY - 1983/12/30
Y1 - 1983/12/30
N2 - A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.
AB - A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.
KW - cloning frequency
KW - limiting dilution cloning
KW - lymphocyte cloning
UR - http://www.scopus.com/inward/record.url?scp=0020972994&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(83)90131-X
DO - 10.1016/0022-1759(83)90131-X
M3 - Article
C2 - 6361146
AN - SCOPUS:0020972994
SN - 0022-1759
VL - 65
SP - 369
EP - 372
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 3
ER -