Cloning of lymphocytes from whole blood by limiting dilution

Kevin J. Trainor, Alexander A. Morley

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)


    A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.

    Original languageEnglish
    Pages (from-to)369-372
    Number of pages4
    JournalJournal of Immunological Methods
    Issue number3
    Publication statusPublished - 30 Dec 1983


    • cloning frequency
    • limiting dilution cloning
    • lymphocyte cloning


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