Cloning of lymphocytes from whole blood by limiting dilution

Kevin J. Trainor; Alexander A. Morley

doi:10.1016/0022-1759(83)90131-X

Cloning of lymphocytes from whole blood by limiting dilution

Kevin J. Trainor
, Alexander A. Morley

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.

Original language	English
Pages (from-to)	369-372
Number of pages	4
Journal	Journal of Immunological Methods
Volume	65
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(83)90131-X
Publication status	Published - 30 Dec 1983

Keywords

cloning frequency
limiting dilution cloning
lymphocyte cloning

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10.1016/0022-1759(83)90131-XLicence: In Copyright

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title = "Cloning of lymphocytes from whole blood by limiting dilution",

abstract = "A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1\%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.",

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AU - Trainor, Kevin J.

AU - Morley, Alexander A.

PY - 1983/12/30

Y1 - 1983/12/30

N2 - A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.

AB - A simple whole blood limiting dilution technique was developed to clone human lymphocytes non-specifically. The geometric mean frequency of clone forming cells in 13 normal individuals was found to be 31.1%. Compared with measurement of proliferation in mass suspension culture, cloning provides a quantitative and easily interpretable endpoint for a variety of lymphocyte studies and its use in measurement of radiation sensitivity is presented as an example.

KW - cloning frequency

KW - limiting dilution cloning

KW - lymphocyte cloning

UR - https://www.scopus.com/pages/publications/0020972994

U2 - 10.1016/0022-1759(83)90131-X

DO - 10.1016/0022-1759(83)90131-X

M3 - Article

C2 - 6361146

AN - SCOPUS:0020972994

SN - 0022-1759

VL - 65

SP - 369

EP - 372

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3

ER -

Cloning of lymphocytes from whole blood by limiting dilution

Abstract

Keywords

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