TY - JOUR
T1 - Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017
AU - Manning, Paul A.
AU - Brown, Melissa H.
AU - Heuzenroeder, Michael W.
PY - 1984/11
Y1 - 1984/11
N2 - We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.
AB - We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.
KW - E. coli K-12
KW - minicells
KW - physical map
KW - protein secretion
KW - Recombinant DNA
KW - transposon mutagenesis
UR - http://www.scopus.com/inward/record.url?scp=0021718292&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(84)90213-0
DO - 10.1016/0378-1119(84)90213-0
M3 - Article
C2 - 6098527
AN - SCOPUS:0021718292
SN - 0378-1119
VL - 31
SP - 225
EP - 231
JO - Gene
JF - Gene
IS - 1-3
ER -