Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017

Paul A. Manning; Melissa H. Brown; Michael W. Heuzenroeder

doi:10.1016/0378-1119(84)90213-0

Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017

Paul A. Manning, Melissa H. Brown, Michael W. Heuzenroeder

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Gene
Volume	31
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(84)90213-0
Publication status	Published - Nov 1984
Externally published	Yes

Keywords

E. coli K-12
minicells
physical map
protein secretion
Recombinant DNA
transposon mutagenesis

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title = "Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017",

abstract = "We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.",

keywords = "E. coli K-12, minicells, physical map, protein secretion, Recombinant DNA, transposon mutagenesis",

author = "Manning, {Paul A.} and Brown, {Melissa H.} and Heuzenroeder, {Michael W.}",

year = "1984",

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doi = "10.1016/0378-1119(84)90213-0",

language = "English",

volume = "31",

pages = "225--231",

journal = "Gene",

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T1 - Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017

AU - Manning, Paul A.

AU - Brown, Melissa H.

AU - Heuzenroeder, Michael W.

PY - 1984/11

Y1 - 1984/11

N2 - We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.

AB - We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified: A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.

KW - E. coli K-12

KW - minicells

KW - physical map

KW - protein secretion

KW - Recombinant DNA

KW - transposon mutagenesis

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U2 - 10.1016/0378-1119(84)90213-0

DO - 10.1016/0378-1119(84)90213-0

M3 - Article

C2 - 6098527

AN - SCOPUS:0021718292

SN - 0378-1119

VL - 31

SP - 225

EP - 231

JO - Gene

JF - Gene

IS - 1-3

ER -

Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017

Abstract

Keywords

UN SDGs

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