Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs

I. L. Gibbins; J. B. Furness; M. Costa; I. MacIntyre; C. J. Hillyard; S. Girgis

doi:10.1016/0304-3940(85)90050-3

Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs

I. L. Gibbins, J. B. Furness, M. Costa, I. MacIntyre, C. J. Hillyard, S. Girgis

Research output: Contribution to journal › Article › peer-review

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Abstract

Calcitonin gene-related peptide (CGRP) has been immunohistochemically co-localized with substance P (SP) in capsaicin-sensitive, varicose axons supplying the skin, viscera and cardiovascular system of the guinea pig. After treatment with colchicine in vitro, 82% of SP neurons in the dorsal root ganglia contained CGRP-like immunoreactivity while 96% of CGRP neurons were immunoreactive for SP. Both CGRP- and SP-like immunoreactive material are transported peripherally and centrally from dorsal root ganglia. Thus, in tissues such as the gut where there are intrinsic nerves containing SP but lacking CGRP, CGRP-like immunoreactivity is a useful means of specifically labelling axons of most sensory neurons containing SP.

Original language	English
Pages (from-to)	125-130
Number of pages	6
Journal	Neuroscience letters
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(85)90050-3
Publication status	Published - 12 Jun 1985

Keywords

calcitonin gene-related peptide
co-localization
guinea pig
immunohistochemistry
sensory nerves
substance P

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title = "Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs",

abstract = "Calcitonin gene-related peptide (CGRP) has been immunohistochemically co-localized with substance P (SP) in capsaicin-sensitive, varicose axons supplying the skin, viscera and cardiovascular system of the guinea pig. After treatment with colchicine in vitro, 82% of SP neurons in the dorsal root ganglia contained CGRP-like immunoreactivity while 96% of CGRP neurons were immunoreactive for SP. Both CGRP- and SP-like immunoreactive material are transported peripherally and centrally from dorsal root ganglia. Thus, in tissues such as the gut where there are intrinsic nerves containing SP but lacking CGRP, CGRP-like immunoreactivity is a useful means of specifically labelling axons of most sensory neurons containing SP.",

keywords = "calcitonin gene-related peptide, co-localization, guinea pig, immunohistochemistry, sensory nerves, substance P",

author = "Gibbins, {I. L.} and Furness, {J. B.} and M. Costa and I. MacIntyre and Hillyard, {C. J.} and S. Girgis",

year = "1985",

month = jun,

day = "12",

doi = "10.1016/0304-3940(85)90050-3",

language = "English",

volume = "57",

pages = "125--130",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier",

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Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs. / Gibbins, I. L.; Furness, J. B.; Costa, M.; MacIntyre, I.; Hillyard, C. J.; Girgis, S.

In: Neuroscience letters, Vol. 57, No. 2, 12.06.1985, p. 125-130.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs

AU - Gibbins, I. L.

AU - Furness, J. B.

AU - Costa, M.

AU - MacIntyre, I.

AU - Hillyard, C. J.

AU - Girgis, S.

PY - 1985/6/12

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N2 - Calcitonin gene-related peptide (CGRP) has been immunohistochemically co-localized with substance P (SP) in capsaicin-sensitive, varicose axons supplying the skin, viscera and cardiovascular system of the guinea pig. After treatment with colchicine in vitro, 82% of SP neurons in the dorsal root ganglia contained CGRP-like immunoreactivity while 96% of CGRP neurons were immunoreactive for SP. Both CGRP- and SP-like immunoreactive material are transported peripherally and centrally from dorsal root ganglia. Thus, in tissues such as the gut where there are intrinsic nerves containing SP but lacking CGRP, CGRP-like immunoreactivity is a useful means of specifically labelling axons of most sensory neurons containing SP.

AB - Calcitonin gene-related peptide (CGRP) has been immunohistochemically co-localized with substance P (SP) in capsaicin-sensitive, varicose axons supplying the skin, viscera and cardiovascular system of the guinea pig. After treatment with colchicine in vitro, 82% of SP neurons in the dorsal root ganglia contained CGRP-like immunoreactivity while 96% of CGRP neurons were immunoreactive for SP. Both CGRP- and SP-like immunoreactive material are transported peripherally and centrally from dorsal root ganglia. Thus, in tissues such as the gut where there are intrinsic nerves containing SP but lacking CGRP, CGRP-like immunoreactivity is a useful means of specifically labelling axons of most sensory neurons containing SP.

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KW - co-localization

KW - guinea pig

KW - immunohistochemistry

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KW - substance P

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