Co-localization of neuropeptide Y, vasoactive intestinal polypeptide and dynorphin in non-noradrenergic axons of the guinea pig uterine artery

J. L. Morris, I. L. Gibbins, J. B. Furness, M. Costa, R. Murphy

    Research output: Contribution to journalArticlepeer-review

    131 Citations (Scopus)

    Abstract

    Two major populations of perivascular axons containing immunoreactivity to neuropeptide Y (NPY) have been revealed in the main uterine artery of the guinea pig by immunohistochemical procedures which allow the simultaneous visualization of two antigens. One population contained immunoreactivity to dopamine-β-hydroxylase (DβH) and was presumably noradrenergic. The other main population of axons with NPY-like immunoreactivity (NPY-LI) did not have DβH-like immunoreactivity (DβH-LI) and was presumably non-noradrenergic. These non-noradrenergic axons also contained immunoreactivity to vasoactive intestinal polypeptide (VIP) and dynorphin (DYN). Indeed, nearly all axons with VIP-LI also contained NPY-LI and DYN-like immunoreactivity (DYN-LI). NPY constricted the uterine artery perfused in vitro, whilst VIP dilated uterine arteries preconstricted with noradrenaline or NPY. Thus, we have evidence for the coexistence of a vasoconstrictor peptide and a vasodilator peptide in the same non-noradrenergic perivascular axons, which also contain an opioid peptide, dynorphin.

    Original languageEnglish
    Pages (from-to)31-37
    Number of pages7
    JournalNeuroscience letters
    Volume62
    Issue number1
    DOIs
    Publication statusPublished - 20 Nov 1985

    Keywords

    • colocalization
    • dynorphin
    • guinea pig
    • immunohistochemistry
    • neuropeptide Y
    • perivascular axon
    • vasoactive intestinal polypeptide

    Fingerprint Dive into the research topics of 'Co-localization of neuropeptide Y, vasoactive intestinal polypeptide and dynorphin in non-noradrenergic axons of the guinea pig uterine artery'. Together they form a unique fingerprint.

    Cite this