TY - JOUR
T1 - Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequencing
AU - Thompson, Fabiano L.
AU - Bruce, Thiago
AU - Gonzalez, Alessandra
AU - Cardoso, Alexander
AU - Clementino, Maysa
AU - Costagliola, Marcela
AU - Hozbor, Constanza
AU - Otero, Ernesto
AU - Piccini, Claudia
AU - Peressutti, Silvia
AU - Schmieder, Robert
AU - Edwards, Robert
AU - Smith, Mathew
AU - Takiyama, Luis Roberto
AU - Vieira, Ricardo
AU - Paranhos, Rodolfo
AU - Artigas, Luis Felipe
PY - 2011/2
Y1 - 2011/2
N2 - The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identified into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a significant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human-contaminated environments may influence bacterioplankton diversity.
AB - The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identified into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a significant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human-contaminated environments may influence bacterioplankton diversity.
KW - Latin America
KW - Marine bacterial diversity
KW - V6 tag pyrosequencing
UR - http://www.scopus.com/inward/record.url?scp=79251642713&partnerID=8YFLogxK
U2 - 10.1007/s00203-010-0644-y
DO - 10.1007/s00203-010-0644-y
M3 - Article
AN - SCOPUS:79251642713
SN - 0302-8933
VL - 193
SP - 105
EP - 114
JO - ARCHIVES OF MICROBIOLOGY
JF - ARCHIVES OF MICROBIOLOGY
IS - 2
ER -