Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequencing

Fabiano L. Thompson, Thiago Bruce, Alessandra Gonzalez, Alexander Cardoso, Maysa Clementino, Marcela Costagliola, Constanza Hozbor, Ernesto Otero, Claudia Piccini, Silvia Peressutti, Robert Schmieder, Robert Edwards, Mathew Smith, Luis Roberto Takiyama, Ricardo Vieira, Rodolfo Paranhos, Luis Felipe Artigas

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identified into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a significant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human-contaminated environments may influence bacterioplankton diversity.

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalARCHIVES OF MICROBIOLOGY
Volume193
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Keywords

  • Latin America
  • Marine bacterial diversity
  • V6 tag pyrosequencing

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