Cold atmospheric plasma for preventing infection of viruses that use ACE2 for entry

Peiyu Wang; Renwu Zhou; Rusen Zhou; Wenshao Li; Janith Weerasinghe; Shuxiong Chen; Bernd H.A. Rehm; Liqian Zhao; Francesca D. Frentiu; Zhifa Zhang; Kexin Yan; Mary Lor; Andreas Suhrbier; Derek J. Richard; Erik W. Thompson; Kostya Ken Ostrikov; Xiaofeng Dai

doi:10.7150/thno.70098

Cold atmospheric plasma for preventing infection of viruses that use ACE2 for entry

Peiyu Wang, Renwu Zhou, Rusen Zhou, Wenshao Li, Janith Weerasinghe, Shuxiong Chen, Bernd H.A. Rehm, Liqian Zhao, Francesca D. Frentiu, Zhifa Zhang, Kexin Yan, Mary Lor, Andreas Suhrbier, Derek J. Richard, Erik W. Thompson, Kostya Ken Ostrikov, Xiaofeng Dai

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Abstract

Rational: The mutating SARS-CoV-2 potentially impairs the efficacy of current vaccines or antibody-based treatments. Broad-spectrum and rapid anti-virus methods feasible for regular epidemic prevention against COVID-19 or alike are urgently called for.

Methods: Using SARS-CoV-2 virus and bioengineered pseudoviruses carrying ACE2-binding spike protein domains, we examined the efficacy of cold atmospheric plasma (CAP) on virus entry prevention.

Results: We found that CAP could effectively inhibit the entry of virus into cells. Direct CAP or CAP-activated medium (PAM) triggered rapid internalization and nuclear translocation of the virus receptor, ACE2, which began to return after 5 hours and was fully recovered by 12 hours. This was seen in vitro with both VERO-E6 cells and human mammary epithelial MCF10A cells, and in vivo. Hydroxyl radical (·OH) and species derived from its interactions with other species were found to be the most effective CAP components for triggering ACE2 nucleus translocation. The ERα/STAT3(Tyr705) and EGFR(Tyr1068/1086)/STAT3(Tyr705) axes were found to interact and collectively mediate the effects on ACE2 localization and expression.

Conclusions: Our data support the use of PAM in helping control SARS-CoV-2 if developed into products for nose/mouth spray; an approach extendable to other viruses utilizing ACE2 for host entry.

Original language	English
Pages (from-to)	2811-2832
Number of pages	22
Journal	Theranostics
Volume	12
Issue number	6
DOIs	https://doi.org/10.7150/thno.70098
Publication status	Published - 2022
Externally published	Yes

Keywords

Angiotensin-converting enzyme 2
cold atmospheric plasma (CAP)
Plasma activated medium (PAM)
SARS-CoV-2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.7150/thno.70098Licence: CC BY

Final published versionFinal published version, 4.05 MBLicence: CC BY

Cite this

@article{bcc73d37da0e4a188cd4b1c6b40bcddd,

title = "Cold atmospheric plasma for preventing infection of viruses that use ACE2 for entry",

abstract = "Rational: The mutating SARS-CoV-2 potentially impairs the efficacy of current vaccines or antibody-based treatments. Broad-spectrum and rapid anti-virus methods feasible for regular epidemic prevention against COVID-19 or alike are urgently called for.Methods: Using SARS-CoV-2 virus and bioengineered pseudoviruses carrying ACE2-binding spike protein domains, we examined the efficacy of cold atmospheric plasma (CAP) on virus entry prevention.Results: We found that CAP could effectively inhibit the entry of virus into cells. Direct CAP or CAP-activated medium (PAM) triggered rapid internalization and nuclear translocation of the virus receptor, ACE2, which began to return after 5 hours and was fully recovered by 12 hours. This was seen in vitro with both VERO-E6 cells and human mammary epithelial MCF10A cells, and in vivo. Hydroxyl radical (·OH) and species derived from its interactions with other species were found to be the most effective CAP components for triggering ACE2 nucleus translocation. The ERα/STAT3(Tyr705) and EGFR(Tyr1068/1086)/STAT3(Tyr705) axes were found to interact and collectively mediate the effects on ACE2 localization and expression.Conclusions: Our data support the use of PAM in helping control SARS-CoV-2 if developed into products for nose/mouth spray; an approach extendable to other viruses utilizing ACE2 for host entry.",

keywords = "Angiotensin-converting enzyme 2, cold atmospheric plasma (CAP), Plasma activated medium (PAM), SARS-CoV-2",

author = "Peiyu Wang and Renwu Zhou and Rusen Zhou and Wenshao Li and Janith Weerasinghe and Shuxiong Chen and Rehm, {Bernd H.A.} and Liqian Zhao and Frentiu, {Francesca D.} and Zhifa Zhang and Kexin Yan and Mary Lor and Andreas Suhrbier and Richard, {Derek J.} and Thompson, {Erik W.} and Ostrikov, {Kostya Ken} and Xiaofeng Dai",

year = "2022",

doi = "10.7150/thno.70098",

language = "English",

volume = "12",

pages = "2811--2832",

journal = "Theranostics",

issn = "1838-7640",

publisher = "Ivyspring International Publisher",

number = "6",

}

TY - JOUR

T1 - Cold atmospheric plasma for preventing infection of viruses that use ACE2 for entry

AU - Wang, Peiyu

AU - Zhou, Renwu

AU - Zhou, Rusen

AU - Li, Wenshao

AU - Weerasinghe, Janith

AU - Chen, Shuxiong

AU - Rehm, Bernd H.A.

AU - Zhao, Liqian

AU - Frentiu, Francesca D.

AU - Zhang, Zhifa

AU - Yan, Kexin

AU - Lor, Mary

AU - Suhrbier, Andreas

AU - Richard, Derek J.

AU - Thompson, Erik W.

AU - Ostrikov, Kostya Ken

AU - Dai, Xiaofeng

PY - 2022

Y1 - 2022

N2 - Rational: The mutating SARS-CoV-2 potentially impairs the efficacy of current vaccines or antibody-based treatments. Broad-spectrum and rapid anti-virus methods feasible for regular epidemic prevention against COVID-19 or alike are urgently called for.Methods: Using SARS-CoV-2 virus and bioengineered pseudoviruses carrying ACE2-binding spike protein domains, we examined the efficacy of cold atmospheric plasma (CAP) on virus entry prevention.Results: We found that CAP could effectively inhibit the entry of virus into cells. Direct CAP or CAP-activated medium (PAM) triggered rapid internalization and nuclear translocation of the virus receptor, ACE2, which began to return after 5 hours and was fully recovered by 12 hours. This was seen in vitro with both VERO-E6 cells and human mammary epithelial MCF10A cells, and in vivo. Hydroxyl radical (·OH) and species derived from its interactions with other species were found to be the most effective CAP components for triggering ACE2 nucleus translocation. The ERα/STAT3(Tyr705) and EGFR(Tyr1068/1086)/STAT3(Tyr705) axes were found to interact and collectively mediate the effects on ACE2 localization and expression.Conclusions: Our data support the use of PAM in helping control SARS-CoV-2 if developed into products for nose/mouth spray; an approach extendable to other viruses utilizing ACE2 for host entry.

AB - Rational: The mutating SARS-CoV-2 potentially impairs the efficacy of current vaccines or antibody-based treatments. Broad-spectrum and rapid anti-virus methods feasible for regular epidemic prevention against COVID-19 or alike are urgently called for.Methods: Using SARS-CoV-2 virus and bioengineered pseudoviruses carrying ACE2-binding spike protein domains, we examined the efficacy of cold atmospheric plasma (CAP) on virus entry prevention.Results: We found that CAP could effectively inhibit the entry of virus into cells. Direct CAP or CAP-activated medium (PAM) triggered rapid internalization and nuclear translocation of the virus receptor, ACE2, which began to return after 5 hours and was fully recovered by 12 hours. This was seen in vitro with both VERO-E6 cells and human mammary epithelial MCF10A cells, and in vivo. Hydroxyl radical (·OH) and species derived from its interactions with other species were found to be the most effective CAP components for triggering ACE2 nucleus translocation. The ERα/STAT3(Tyr705) and EGFR(Tyr1068/1086)/STAT3(Tyr705) axes were found to interact and collectively mediate the effects on ACE2 localization and expression.Conclusions: Our data support the use of PAM in helping control SARS-CoV-2 if developed into products for nose/mouth spray; an approach extendable to other viruses utilizing ACE2 for host entry.

KW - Angiotensin-converting enzyme 2

KW - cold atmospheric plasma (CAP)

KW - Plasma activated medium (PAM)

KW - SARS-CoV-2

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DO - 10.7150/thno.70098

M3 - Article

C2 - 35401827

AN - SCOPUS:85127530435

SN - 1838-7640

VL - 12

SP - 2811

EP - 2832

JO - Theranostics

JF - Theranostics

IS - 6

ER -

Cold atmospheric plasma for preventing infection of viruses that use ACE2 for entry

Abstract

Keywords

UN SDGs

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