Combined Systems Approaches Reveal Highly Plastic Responses to Antimicrobial Peptide Challenge in Escherichia coli

Justyna Kozlowska, Louic Vermeer, Geraint Rogers, Nabila Rehnnuma, Sarah-Beth Amos, Garrit Koller, Michael McArthur, Kenneth Bruce, James Mason

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Obtaining an in-depth understanding of the arms races between peptides comprising the innate immune response and bacterial pathogens is of fundamental interest and will inform the development of new antibacterial therapeutics. We investigated whether a whole organism view of antimicrobial peptide (AMP) challenge on Escherichia coli would provide a suitably sophisticated bacterial perspective on AMP mechanism of action. Selecting structurally and physically related AMPs but with expected differences in bactericidal strategy, we monitored changes in bacterial metabolomes, morphological features and gene expression following AMP challenge at sub-lethal concentrations. For each technique, the vast majority of changes were specific to each AMP, with such a plastic response indicating E. coli is highly capable of discriminating between specific antibiotic challenges. Analysis of the ontological profiles generated from the transcriptomic analyses suggests this approach can accurately predict the antibacterial mode of action, providing a fresh, novel perspective for previous functional and biophysical studies.

    Original languageEnglish
    Article numbere1004104
    Pages (from-to)e1004104-e1004104
    JournalPLoS Pathogens
    Volume10
    Issue number5
    DOIs
    Publication statusPublished - May 2014

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