Comparable performance characteristics of plasma thiamine and erythrocyte thiamine diphosphate in response to thiamine fortification in rural Cambodian women

Adrian McCann; Øivind Midttun; Kyly C. Whitfield; Hou Kroeun; Mam Borath; Prak Sophonneary; Per Magne Ueland; Timothy J. Green

doi:10.3390/nu9070676

Comparable performance characteristics of plasma thiamine and erythrocyte thiamine diphosphate in response to thiamine fortification in rural Cambodian women

Adrian McCann, Øivind Midttun, Kyly C. Whitfield, Hou Kroeun, Mam Borath, Prak Sophonneary, Per Magne Ueland, Timothy J. Green

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Abstract

Background: Traditionally, vitamin B₁ status is assessed by a functional test measuring erythrocyte transketolase (ETK) activity or direct measurement of erythrocyte thiamine diphosphate (eThDP) concentration. However, such analyses are logistically challenging, and do not allow assessment of vitamin B₁ status in plasma/serum samples stored in biobanks. Using a multiplex assay, we evaluated plasma concentrations of thiamine and thiamine monophosphate (TMP), as alternative, convenient measures of vitamin B₁ status. Methods: We investigated the relationships between the established biomarker eThDP and plasma concentrations of thiamine and TMP, and compared the response of these thiamine forms to thiamine fortification using samples from 196 healthy Cambodian women (aged 18–45 years.). eThDP was measured by high performance liquid chromatography with fluorescence detection (HPLC-FLD) and plasma thiamine and TMP by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Plasma thiamine and TMP correlated significantly with eThDP at baseline and study-end (p < 0.05). Among the fortification groups, the strongest response was observed for plasma thiamine (increased by 266%), while increases in plasma TMP (60%) and eThDP (53%) were comparable. Conclusions: Plasma thiamine and TMP correlated positively with eThDP, and all thiamine forms responded significantly to thiamine intervention. Measuring plasma concentrations of thiamine forms is advantageous due to convenient sample handling and capacity to develop low volume, high-throughput, multiplex assays.

Original language	English
Article number	676
Number of pages	12
Journal	Nutrients
Volume	9
Issue number	7
DOIs	https://doi.org/10.3390/nu9070676
Publication status	Published - Jul 2017
Externally published	Yes

Bibliographical note

Funding Information:
Work supported by Grand Challenges Canada Stars in Global Health (S6 0490-01-10). K.C.W. is supported by the International Development Research Centre Doctoral Research Award, and the Canadian Institutes of Health Research Doctoral Award—Frederick Banting and Charles Best Canada Graduate Scholarship (327933). Fish sauce production: Natural Factors (Coquitlam, BC, Canada) donated the thiamine hydrochloride, and Leang Lang Enterprises produced the thiamine-fortified fish sauce. The work undertaken by the authors was in part supported by the Foundation to Support Research in Functional Vitamin B12 Deficiency, Bergen, Norway.

Publisher Copyright:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Direct biomarkers
Erythrocyte thiamine diphosphate
Plasma
Thiamine (vitamin B)
Thiamine monophospahte
Cambodian women

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Cite this

@article{e2684210ba614e4e95385ddb3602db67,

title = "Comparable performance characteristics of plasma thiamine and erythrocyte thiamine diphosphate in response to thiamine fortification in rural Cambodian women",

abstract = "Background: Traditionally, vitamin B1 status is assessed by a functional test measuring erythrocyte transketolase (ETK) activity or direct measurement of erythrocyte thiamine diphosphate (eThDP) concentration. However, such analyses are logistically challenging, and do not allow assessment of vitamin B1 status in plasma/serum samples stored in biobanks. Using a multiplex assay, we evaluated plasma concentrations of thiamine and thiamine monophosphate (TMP), as alternative, convenient measures of vitamin B1 status. Methods: We investigated the relationships between the established biomarker eThDP and plasma concentrations of thiamine and TMP, and compared the response of these thiamine forms to thiamine fortification using samples from 196 healthy Cambodian women (aged 18–45 years.). eThDP was measured by high performance liquid chromatography with fluorescence detection (HPLC-FLD) and plasma thiamine and TMP by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Plasma thiamine and TMP correlated significantly with eThDP at baseline and study-end (p < 0.05). Among the fortification groups, the strongest response was observed for plasma thiamine (increased by 266%), while increases in plasma TMP (60%) and eThDP (53%) were comparable. Conclusions: Plasma thiamine and TMP correlated positively with eThDP, and all thiamine forms responded significantly to thiamine intervention. Measuring plasma concentrations of thiamine forms is advantageous due to convenient sample handling and capacity to develop low volume, high-throughput, multiplex assays.",

keywords = "Direct biomarkers, Erythrocyte thiamine diphosphate, Plasma, Thiamine (vitamin B), Thiamine monophospahte, Cambodian women",

author = "Adrian McCann and {\O}ivind Midttun and Whitfield, {Kyly C.} and Hou Kroeun and Mam Borath and Prak Sophonneary and Ueland, {Per Magne} and Green, {Timothy J.}",

note = "Funding Information: Work supported by Grand Challenges Canada Stars in Global Health (S6 0490-01-10). K.C.W. is supported by the International Development Research Centre Doctoral Research Award, and the Canadian Institutes of Health Research Doctoral Award—Frederick Banting and Charles Best Canada Graduate Scholarship (327933). Fish sauce production: Natural Factors (Coquitlam, BC, Canada) donated the thiamine hydrochloride, and Leang Lang Enterprises produced the thiamine-fortified fish sauce. The work undertaken by the authors was in part supported by the Foundation to Support Research in Functional Vitamin B12 Deficiency, Bergen, Norway. Publisher Copyright: {\textcopyright} 2017 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2017",

month = jul,

doi = "10.3390/nu9070676",

language = "English",

volume = "9",

journal = "Nutrients",

issn = "2072-6643",

publisher = "MDPI",

number = "7",

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T1 - Comparable performance characteristics of plasma thiamine and erythrocyte thiamine diphosphate in response to thiamine fortification in rural Cambodian women

AU - McCann, Adrian

AU - Midttun, Øivind

AU - Whitfield, Kyly C.

AU - Kroeun, Hou

AU - Borath, Mam

AU - Sophonneary, Prak

AU - Ueland, Per Magne

AU - Green, Timothy J.

N1 - Funding Information: Work supported by Grand Challenges Canada Stars in Global Health (S6 0490-01-10). K.C.W. is supported by the International Development Research Centre Doctoral Research Award, and the Canadian Institutes of Health Research Doctoral Award—Frederick Banting and Charles Best Canada Graduate Scholarship (327933). Fish sauce production: Natural Factors (Coquitlam, BC, Canada) donated the thiamine hydrochloride, and Leang Lang Enterprises produced the thiamine-fortified fish sauce. The work undertaken by the authors was in part supported by the Foundation to Support Research in Functional Vitamin B12 Deficiency, Bergen, Norway. Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2017/7

Y1 - 2017/7

N2 - Background: Traditionally, vitamin B1 status is assessed by a functional test measuring erythrocyte transketolase (ETK) activity or direct measurement of erythrocyte thiamine diphosphate (eThDP) concentration. However, such analyses are logistically challenging, and do not allow assessment of vitamin B1 status in plasma/serum samples stored in biobanks. Using a multiplex assay, we evaluated plasma concentrations of thiamine and thiamine monophosphate (TMP), as alternative, convenient measures of vitamin B1 status. Methods: We investigated the relationships between the established biomarker eThDP and plasma concentrations of thiamine and TMP, and compared the response of these thiamine forms to thiamine fortification using samples from 196 healthy Cambodian women (aged 18–45 years.). eThDP was measured by high performance liquid chromatography with fluorescence detection (HPLC-FLD) and plasma thiamine and TMP by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Plasma thiamine and TMP correlated significantly with eThDP at baseline and study-end (p < 0.05). Among the fortification groups, the strongest response was observed for plasma thiamine (increased by 266%), while increases in plasma TMP (60%) and eThDP (53%) were comparable. Conclusions: Plasma thiamine and TMP correlated positively with eThDP, and all thiamine forms responded significantly to thiamine intervention. Measuring plasma concentrations of thiamine forms is advantageous due to convenient sample handling and capacity to develop low volume, high-throughput, multiplex assays.

AB - Background: Traditionally, vitamin B1 status is assessed by a functional test measuring erythrocyte transketolase (ETK) activity or direct measurement of erythrocyte thiamine diphosphate (eThDP) concentration. However, such analyses are logistically challenging, and do not allow assessment of vitamin B1 status in plasma/serum samples stored in biobanks. Using a multiplex assay, we evaluated plasma concentrations of thiamine and thiamine monophosphate (TMP), as alternative, convenient measures of vitamin B1 status. Methods: We investigated the relationships between the established biomarker eThDP and plasma concentrations of thiamine and TMP, and compared the response of these thiamine forms to thiamine fortification using samples from 196 healthy Cambodian women (aged 18–45 years.). eThDP was measured by high performance liquid chromatography with fluorescence detection (HPLC-FLD) and plasma thiamine and TMP by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Plasma thiamine and TMP correlated significantly with eThDP at baseline and study-end (p < 0.05). Among the fortification groups, the strongest response was observed for plasma thiamine (increased by 266%), while increases in plasma TMP (60%) and eThDP (53%) were comparable. Conclusions: Plasma thiamine and TMP correlated positively with eThDP, and all thiamine forms responded significantly to thiamine intervention. Measuring plasma concentrations of thiamine forms is advantageous due to convenient sample handling and capacity to develop low volume, high-throughput, multiplex assays.

KW - Direct biomarkers

KW - Erythrocyte thiamine diphosphate

KW - Plasma

KW - Thiamine (vitamin B)

KW - Thiamine monophospahte

KW - Cambodian women

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DO - 10.3390/nu9070676

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AN - SCOPUS:85022049339

SN - 2072-6643

VL - 9

JO - Nutrients

JF - Nutrients

IS - 7

M1 - 676

ER -

Comparable performance characteristics of plasma thiamine and erythrocyte thiamine diphosphate in response to thiamine fortification in rural Cambodian women

Abstract

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Keywords

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