Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies

Carmela Ricciardelli, Tina Bianco-Miotto, Shalini Jindal, Thomas Dodd, Penelope Cohen, Villis Marshall, Peter Sutherland, Hemamali Samaratunga, James Kench, Ying Dong, Hong Wang, Judith Clements, Gail Risbridger, Robert Sutherland, Wayne Tilley, David Horsfall, Australian Prostate Cancer Bio

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Background: Knowledge of preanalytic conditions that biospecimens are subjected to is critically important because novel surgical procedures, tissue sampling, handling, and storage might affect biomarker expression or invalidate tissue samples as analytes for some technologies. Methods: We investigated differences in RNA quality, gene expression by quantitative real-time PCR, and immunoreactive protein expression of selected prostate cancer biomarkers between tissues from retropubic radical prostatectomy(RRP) and robot-assisted laparoscopic prostatectomy (RALP). Sections of tissue microarray of 23 RALP and 22 RRP samples were stained with antibodies to androgen receptor (AR) and prostate-specific antigen (PSA) as intersite controls, and 14 other candidate biomarkers of research interest to three laboratories within the Australian Prostate Cancer BioResource tissue banking network. Quantitative real-time PCRwas done for AR, PSA (KLK3), KLK2, KLK4, and HIF1A on RNAextracted from five RALP and five RRP frozen tissue cores. Results: No histologic differences were observed between RALP and RRP tissue. Biomarker staining grouped these samples into those with increased (PSA, CK8/18, CKHMW, KLK4), decreased (KLK2, KLK14), or no change in expression (AR, ghrelin, Ki67, PCNA, VEGF-C, PAR2, YB1, p63, versican, and chondroitin 0-sulfate) in RALP compared with RRP tissue. No difference in RNA quality or gene expression was detected between RALP and RRP tissue. Conclusions: Changes in biomarker expression between RALP and RRP tissue exist at the immunoreactive protein level, but the etiology is unclear. Impact: Future studies should account for changes in biomarker expression when using RALP tissues, and mixed cohorts of RALP and RRP tissue should be avoided.

    Original languageEnglish
    Pages (from-to)1755-1765
    Number of pages11
    JournalCancer Epidemiology Biomarkers and Prevention
    Volume19
    Issue number7
    DOIs
    Publication statusPublished - Jul 2010

    Fingerprint Dive into the research topics of 'Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies'. Together they form a unique fingerprint.

  • Cite this

    Ricciardelli, C., Bianco-Miotto, T., Jindal, S., Dodd, T., Cohen, P., Marshall, V., Sutherland, P., Samaratunga, H., Kench, J., Dong, Y., Wang, H., Clements, J., Risbridger, G., Sutherland, R., Tilley, W., Horsfall, D., & Australian Prostate Cancer Bio (2010). Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies. Cancer Epidemiology Biomarkers and Prevention, 19(7), 1755-1765. https://doi.org/10.1158/1055-9965.EPI-10-0059