Comparative genome analysis proposes three new Aureobasidium species isolated from grape juice

Cristobal A. Onetto, Simon A. Schmidt, Michael J. Roach, Anthony R. Borneman

Research output: Contribution to journalArticlepeer-review

Abstract

Aureobasidium pullulans is the most abundant and ubiquitous species within the genus and is also considered a core component of the grape juice microflora. So far, a small number of other Aureobasidium species have been reported, that in contrast to A. pullulans, appear far more constrained to specific habitats. It is unknown whether grape juice is a reservoir of novel Aureobasidium species, overlooked in the course of conventional morphological and meta-barcoding analyses. In this study, eight isolates from grape juice taxonomically classified as Aureobasidium through ITS sequencing were subjected to whole-genome phylogenetic, synteny and nucleotide identity analyses, which revealed three isolates to likely represent newly discovered Aureobasidium species. Analyses of ITS and metagenomic sequencing datasets show that these species can be present in grape juice samples from different locations and vintages. Functional annotation revealed the Aureobasidium isolates possess the genetic potential to support growth on the surface of plants and grapes. However, the loss of several genes associated with tolerance to diverse environmental stresses suggest a more constrained ecological range than A. pullulans.

Original languageEnglish
Article numberfoaa052
Number of pages16
JournalFEMS YEAST RESEARCH
Volume20
Issue number6
DOIs
Publication statusPublished - Sep 2020
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by Australian grapegrowers and winemakers through their investment body Wine Australia, with matching funds from the Australian Government. The AWRI is a member of the Wine Innovation Cluster (WIC) in Adelaide.

Publisher Copyright:
© FEMS 2020. All rights reserved.

Keywords

  • Aureobasidium
  • Comparative genomics
  • Grape juice
  • Metagenomics
  • Wine

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