Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications

Silvina  Dayer; Johannes D. Scharwies; Sunita A. Ramesh; Wendy Sullivan; Franziska C. Doerflinger; Vinay Pagay; Stephen D. Tyerman

doi:10.3389/fpls.2020.00705

Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications

Silvina Dayer, Johannes D. Scharwies, Sunita A. Ramesh, Wendy Sullivan, Franziska C. Doerflinger, Vinay Pagay, Stephen D. Tyerman

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Abstract

Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions.

Original language	English
Article number	705
Number of pages	14
Journal	Frontiers in Plant Science
Volume	11
DOIs	https://doi.org/10.3389/fpls.2020.00705
Publication status	Published - 19 Jun 2020
Externally published	Yes

Bibliographical note

Copyright © 2020 Dayer, Scharwies, Ramesh, Sullivan, Doerflinger, Pagay and
Tyerman. This is an open-access article distributed under the terms of the Creative
Commons Attribution License (CC BY). The use, distribution or reproduction in
other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

Vitis vinifera
aquaporin
gas exchange
hydraulic conductivity
isohydric
isohydrodynamic
plasma membrane intrinsic protein
tonoplast intrinsic protein

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10.3389/fpls.2020.00705Licence: CC BY

Published versionFinal published version, 2.1 MBLicence: CC BY

Cite this

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title = "Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications",

abstract = "Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions.",

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AU - Tyerman, Stephen D.

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N2 - Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions.

AB - Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions.

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KW - hydraulic conductivity

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KW - isohydrodynamic

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KW - tonoplast intrinsic protein

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DO - 10.3389/fpls.2020.00705

M3 - Article

SN - 1664-462X

VL - 11

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 705

ER -

Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications

Abstract

Bibliographical note

Keywords

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