Comparison of cell disruption methods for improving lipid extraction from thraustochytrid strains

Avinesh Byreddy, Adarsha Gupta, Colin Barrow, Munish Puri

    Research output: Contribution to journalArticlepeer-review

    87 Citations (Scopus)

    Abstract

    Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production.

    Original languageEnglish
    Pages (from-to)5111-5127
    Number of pages17
    JournalMarine Drugs
    Volume13
    Issue number8
    DOIs
    Publication statusPublished - 1 Aug 2015

    Keywords

    • Algae
    • Biodiesel
    • DHA
    • Marine
    • Omega-3
    • Solvents

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