Comparison of ELISA and RT-PCR for the detection of PNRSV and PDV in Australian almond trees

Genet Mekuria, Sunita Ramesh, Evita Alberts, Terry Bertozzi, M. Wirthensohn, Graham Collins, M. Sedgley

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This three-year study compared the use of ELISA and RT-PCR for identifying Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) on 175 leaf samples taken from trees maintained as a budwood repository for almond growers. The primers used for RT-PCR were based on DNA sequences that code for coat protein, and both viruses could be detected in the same reaction. For PNRSV, both ELISA and RT-PCR produced similar results, although RT-PCR was more consistent, and had the added advantage that plant material could be tested at any time throughout the growing season. For PDV, virus particles were not detected by ELISA, but were detected in low titre using a nested PCR technique. RT-PCR is used routinely now to index progeny developed each year by the Australian almond improvement program in place of both graft incompatibility using woody indicator species and ELISA.
Original languageEnglish
Title of host publicationXIII GREMPA meeting on almonds and pistachios
Place of PublicationZaragoza, Spain
PublisherInstituto Agronomico Mediterraneo
Pages193-196
ISBN (Print)2853523071 , 9782853523073
Publication statusPublished - 2005
Externally publishedYes

Publication series

NameOptions Méditerranéennes. serie A : séminaires Méditerrannéens
PublisherInstituto Agronomico Mediterraneo
Number63

Keywords

  • Prunus
  • almond
  • PNRSV
  • PDV
  • ELISA
  • RT-PCR

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