Abstract
A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.
Original language | English |
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Pages (from-to) | 65-69 |
Number of pages | 5 |
Journal | Journal of Virological Methods |
Volume | 114 |
Issue number | 1 |
DOIs | |
Publication status | Published - Dec 2003 |
Externally published | Yes |
Keywords
- ELISA
- Ilarvirus
- Prune dwarf virus
- Prunus necrotic ringspot virus
- RT-PCR