Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

Genet Mekuria; Sunita A. Ramesh; Evita Alberts; Terry Bertozzi; Michelle Wirthensohn; Graham Collins; Margaret Sedgley

doi:10.1016/j.jviromet.2003.08.014

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

Genet Mekuria, Sunita A. Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins, Margaret Sedgley

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

Original language	English
Pages (from-to)	65-69
Number of pages	5
Journal	Journal of Virological Methods
Volume	114
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2003.08.014
Publication status	Published - Dec 2003
Externally published	Yes

Keywords

ELISA
Ilarvirus
Prune dwarf virus
Prunus necrotic ringspot virus
RT-PCR

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@article{49c69ef566584574a4351c2f25c9bd4e,

title = "Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)",

abstract = "A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.",

keywords = "ELISA, Ilarvirus, Prune dwarf virus, Prunus necrotic ringspot virus, RT-PCR",

author = "Genet Mekuria and Ramesh, {Sunita A.} and Evita Alberts and Terry Bertozzi and Michelle Wirthensohn and Graham Collins and Margaret Sedgley",

year = "2003",

month = dec,

doi = "10.1016/j.jviromet.2003.08.014",

language = "English",

volume = "114",

pages = "65--69",

journal = "Journal of Virological Methods",

issn = "0166-0934",

number = "1",

}

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis). / Mekuria, Genet; Ramesh, Sunita A.; Alberts, Evita; Bertozzi, Terry; Wirthensohn, Michelle; Collins, Graham; Sedgley, Margaret.

In: Journal of Virological Methods, Vol. 114, No. 1, 12.2003, p. 65-69.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

AU - Mekuria, Genet

AU - Ramesh, Sunita A.

AU - Alberts, Evita

AU - Bertozzi, Terry

AU - Wirthensohn, Michelle

AU - Collins, Graham

AU - Sedgley, Margaret

PY - 2003/12

Y1 - 2003/12

N2 - A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

AB - A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

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