Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

Genet Mekuria, Sunita A. Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins, Margaret Sedgley

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalJournal of Virological Methods
Volume114
Issue number1
DOIs
Publication statusPublished - Dec 2003
Externally publishedYes

Keywords

  • ELISA
  • Ilarvirus
  • Prune dwarf virus
  • Prunus necrotic ringspot virus
  • RT-PCR

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