TY - JOUR
T1 - Comparison of human serum dopamine β hydroxylase levels by radioimmunoassay and enzymatic assay
AU - Rush, R. A.
AU - Thomas, P. E.
AU - Nagatsu, T.
AU - Udenfriend, S.
PY - 1974/3
Y1 - 1974/3
N2 - Human serum dopamine β hydroxylase (3,4 dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1) was measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine β hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3 fold range: from 11.7 μg/ml to 34.4 μg/ml. From an analysis of the purified enzyme, the percentage of serum dopamine β hydroxylase that was catalytically active was estimated to be small and variable.
AB - Human serum dopamine β hydroxylase (3,4 dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1) was measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine β hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3 fold range: from 11.7 μg/ml to 34.4 μg/ml. From an analysis of the purified enzyme, the percentage of serum dopamine β hydroxylase that was catalytically active was estimated to be small and variable.
UR - http://www.scopus.com/inward/record.url?scp=0016381869&partnerID=8YFLogxK
U2 - 10.1073/pnas.71.3.872
DO - 10.1073/pnas.71.3.872
M3 - Article
C2 - 4522796
AN - SCOPUS:0016381869
SN - 0027-8424
VL - 71
SP - 872
EP - 874
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3
ER -