Comparison of human serum dopamine β hydroxylase levels by radioimmunoassay and enzymatic assay

R. A. Rush, P. E. Thomas, T. Nagatsu, S. Udenfriend

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Human serum dopamine β hydroxylase (3,4 dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1) was measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine β hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3 fold range: from 11.7 μg/ml to 34.4 μg/ml. From an analysis of the purified enzyme, the percentage of serum dopamine β hydroxylase that was catalytically active was estimated to be small and variable.

Original languageEnglish
Pages (from-to)872-874
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number3
DOIs
Publication statusPublished - Mar 1974
Externally publishedYes

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