Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

Mirjam Kaestli; Leisha Richardson; Rebecca Colman; Apichai Tuanyok; Erin Price; Jolene Bowers; Mark Mayo; Erin Kelley; Meagan Seymour; Derek Sarovich; Talima Pearson; David Engelthaler; David Wagner; Paul Keim; James Schupp; Bart Currie

doi:10.1128/JCM.06737-11

Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

Mirjam Kaestli, Leisha Richardson, Rebecca Colman, Apichai Tuanyok, Erin Price, Jolene Bowers, Mark Mayo, Erin Kelley, Meagan Seymour, Derek Sarovich, Talima Pearson, David Engelthaler, David Wagner, Paul Keim, James Schupp, Bart Currie

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35 Citations (Scopus)

Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

Original language	English
Pages (from-to)	2059-2062
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1128/JCM.06737-11
Publication status	Published - Jun 2012

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Kaestli, M., Richardson, L., Colman, R., Tuanyok, A., Price, E., Bowers, J., Mayo, M., Kelley, E., Seymour, M., Sarovich, D., Pearson, T., Engelthaler, D., Wagner, D., Keim, P., Schupp, J., & Currie, B. (2012). Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens. Journal of Clinical Microbiology, 50(6), 2059-2062. https://doi.org/10.1128/JCM.06737-11

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title = "Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens",

abstract = "Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.",

author = "Mirjam Kaestli and Leisha Richardson and Rebecca Colman and Apichai Tuanyok and Erin Price and Jolene Bowers and Mark Mayo and Erin Kelley and Meagan Seymour and Derek Sarovich and Talima Pearson and David Engelthaler and David Wagner and Paul Keim and James Schupp and Bart Currie",

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doi = "10.1128/JCM.06737-11",

language = "English",

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Kaestli, M, Richardson, L, Colman, R, Tuanyok, A, Price, E, Bowers, J, Mayo, M, Kelley, E, Seymour, M, Sarovich, D, Pearson, T, Engelthaler, D, Wagner, D, Keim, P, Schupp, J & Currie, B 2012, 'Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens', Journal of Clinical Microbiology, vol. 50, no. 6, pp. 2059-2062. https://doi.org/10.1128/JCM.06737-11

TY - JOUR

T1 - Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

AU - Kaestli, Mirjam

AU - Richardson, Leisha

AU - Colman, Rebecca

AU - Tuanyok, Apichai

AU - Price, Erin

AU - Bowers, Jolene

AU - Mayo, Mark

AU - Kelley, Erin

AU - Seymour, Meagan

AU - Sarovich, Derek

AU - Pearson, Talima

AU - Engelthaler, David

AU - Wagner, David

AU - Keim, Paul

AU - Schupp, James

AU - Currie, Bart

PY - 2012/6

Y1 - 2012/6

N2 - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

AB - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

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U2 - 10.1128/JCM.06737-11

DO - 10.1128/JCM.06737-11

M3 - Article

SN - 0095-1137

VL - 50

SP - 2059

EP - 2062

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

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