TY - JOUR
T1 - Construction of an ~ 700-kb transcript map around the familial Mediterranean fever locus on human chromosome 16p13.3
AU - Centola, Michael
AU - Chen, Xiaoguang
AU - Sood, Raman
AU - Deng, Zuoming
AU - Aksentijevich, Ivona
AU - Blake, Trevor
AU - Ricke, Darrell O.
AU - Chen, Xiang
AU - Wood, Geryl
AU - Zaks, Nurit
AU - Richards, Neil
AU - Krizman, David
AU - Mansfield, Elizabeth
AU - Apostolou, Sinoula
AU - Liu, Jingmei
AU - Shafran, Neta
AU - Vedula, Anil
AU - Hamon, Melanie
AU - Cercek, Andrea
AU - Kahan, Tanaz
AU - Gumucio, Deborah
AU - Callen, David F.
AU - Richards, Robert I.
AU - Moyzis, Robert K.
AU - Doggett, Norman A.
AU - Collins, Francis S.
AU - Paul Liu, P.
AU - Fischel-Ghodsian, Nathan
AU - Kastner, Daniel L.
PY - 1998
Y1 - 1998
N2 - We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ~ 700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron-exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (between D16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.
AB - We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ~ 700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron-exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (between D16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.
UR - https://www.scopus.com/pages/publications/0032201126
U2 - 10.1101/gr.8.11.1172
DO - 10.1101/gr.8.11.1172
M3 - Article
C2 - 9847080
AN - SCOPUS:0032201126
SN - 1088-9051
VL - 8
SP - 1172
EP - 1191
JO - Genome Research
JF - Genome Research
IS - 11
ER -