Contributions to a better comprehension of redox-mediated decolouration and detoxification of azo dyes by a laccase produced by Streptomyces cyaneus CECT 3335

Raquel Moya, M Hernandez, Ana Garcia-Martin, Andrew Ball, M. Enriqueta Arias

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    The ability of a laccase (EC 1.10.3.2) produced by Streptomyces cyaneus CECT 3335 to decolourise and detoxify azo dyes was assessed. Results showed that a colour loss of 90% was achieved only in the presence of acetosyringone (0.1 mM) acting as a redox mediator for the laccase. Toxicological analysis of the decolourised dyes revealed that there was no direct correlation between decolouration and detoxification; in fact, in the case of the dyes Methyl Orange and Orange II, a significant increase in toxicity was produced after the treatment. In contrast, a significant decrease in toxicity was observed after the decolouration of New Coccine and Chromotrope 2R. Finally, HPLC analysis of the dyes after treatment revealed the complete disappearance of both dyes and mediator and a concomitant appearance of new chromatographic peaks which could be responsible of the residual toxicity detected in some cases.

    Original languageEnglish
    Pages (from-to)2224-2229
    Number of pages6
    JournalBioresource Technology
    Volume101
    Issue number7
    DOIs
    Publication statusPublished - Apr 2010

    Keywords

    • Azo dyes
    • Laccase
    • Natural mediator
    • Streptomyces cyaneus
    • Toxicity

    Fingerprint Dive into the research topics of 'Contributions to a better comprehension of redox-mediated decolouration and detoxification of azo dyes by a laccase produced by Streptomyces cyaneus CECT 3335'. Together they form a unique fingerprint.

  • Cite this