CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique

Amanda Choo; Elisabeth Fung; Thu N.M. Nguyen; Anzu Okada; Peter Crisp

doi:10.1007/978-1-0716-2301-5_9

CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique

Amanda Choo, Elisabeth Fung, Thu N.M. Nguyen, Anzu Okada, Peter Crisp

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Citations (Scopus)

Abstract

Sterile Insect Technique (SIT) is a biocontrol strategy that has been widely utilized to suppress or eradicate outbreak populations of insect pests such as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Development of these desirable strains has been facilitated by the emergence of the CRISPR/Cas genome-editing technology that enables the rapid and precise genomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest Bactrocera tryoni (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.

Original language	English
Title of host publication	Applications of Genome Modulation and Editing
Editors	Paul John Verma, Huseyin Sumer, Jun Liu
Place of Publication	New York, USA
Publisher	Humana Press
Chapter	9
Pages	151-171
Number of pages	21
ISBN (Electronic)	978-1-0716-2301-5
ISBN (Print)	978-1-0716-2300-8
DOIs	https://doi.org/10.1007/978-1-0716-2301-5_9
Publication status	Published - 2022
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2495
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Bactrocera tryoni
CRISPR/Cas mutagenesis
Embryo microinjections
Genetic sexing strains
Queensland fruit fly
Sterile insect technique
Tephritids

Access to Document

10.1007/978-1-0716-2301-5_9Licence: In Copyright

Cite this

Choo, A., Fung, E., Nguyen, T. N. M., Okada, A., & Crisp, P. (2022). CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique. In P. J. Verma, H. Sumer, & J. Liu (Eds.), Applications of Genome Modulation and Editing (pp. 151-171). (Methods in Molecular Biology; Vol. 2495). Humana Press. https://doi.org/10.1007/978-1-0716-2301-5_9

@inbook{74ce3d25106b4eaea8e5f12a5ac8617e,

title = "CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique",

abstract = "Sterile Insect Technique (SIT) is a biocontrol strategy that has been widely utilized to suppress or eradicate outbreak populations of insect pests such as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Development of these desirable strains has been facilitated by the emergence of the CRISPR/Cas genome-editing technology that enables the rapid and precise genomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest Bactrocera tryoni (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.",

keywords = "Bactrocera tryoni, CRISPR/Cas mutagenesis, Embryo microinjections, Genetic sexing strains, Queensland fruit fly, Sterile insect technique, Tephritids",

author = "Amanda Choo and Elisabeth Fung and Nguyen, {Thu N.M.} and Anzu Okada and Peter Crisp",

year = "2022",

doi = "10.1007/978-1-0716-2301-5_9",

language = "English",

isbn = "978-1-0716-2300-8",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "151--171",

editor = "Verma, {Paul John } and Sumer, {Huseyin } and Liu, {Jun }",

booktitle = "Applications of Genome Modulation and Editing",

address = "United States",

}

CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique. / Choo, Amanda; Fung, Elisabeth; Nguyen, Thu N.M. et al.
Applications of Genome Modulation and Editing. ed. / Paul John Verma; Huseyin Sumer; Jun Liu. New York, USA: Humana Press, 2022. p. 151-171 (Methods in Molecular Biology; Vol. 2495).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique

AU - Choo, Amanda

AU - Fung, Elisabeth

AU - Nguyen, Thu N.M.

AU - Okada, Anzu

AU - Crisp, Peter

PY - 2022

Y1 - 2022

N2 - Sterile Insect Technique (SIT) is a biocontrol strategy that has been widely utilized to suppress or eradicate outbreak populations of insect pests such as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Development of these desirable strains has been facilitated by the emergence of the CRISPR/Cas genome-editing technology that enables the rapid and precise genomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest Bactrocera tryoni (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.

AB - Sterile Insect Technique (SIT) is a biocontrol strategy that has been widely utilized to suppress or eradicate outbreak populations of insect pests such as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Development of these desirable strains has been facilitated by the emergence of the CRISPR/Cas genome-editing technology that enables the rapid and precise genomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest Bactrocera tryoni (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.

KW - Bactrocera tryoni

KW - CRISPR/Cas mutagenesis

KW - Embryo microinjections

KW - Genetic sexing strains

KW - Queensland fruit fly

KW - Sterile insect technique

KW - Tephritids

UR - http://www.scopus.com/inward/record.url?scp=85132809549&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2301-5_9

DO - 10.1007/978-1-0716-2301-5_9

M3 - Chapter

C2 - 35696033

AN - SCOPUS:85132809549

SN - 978-1-0716-2300-8

T3 - Methods in Molecular Biology

SP - 151

EP - 171

BT - Applications of Genome Modulation and Editing

A2 - Verma, Paul John

A2 - Sumer, Huseyin

A2 - Liu, Jun

PB - Humana Press

CY - New York, USA

ER -

CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique

Abstract

Publication series

Keywords

Access to Document

Other files and links

Fingerprint

Cite this