Abstract
Methods for assembly, taxonomic profiling and binning are key to interpreting metagenome data, but a lack of consensus about benchmarking complicates performance assessment. The Critical Assessment of Metagenome Interpretation (CAMI) challenge has engaged the global developer community to benchmark their programs on highly complex and realistic data sets, generated from ∼700 newly sequenced microorganisms and ∼600 novel viruses and plasmids and representing common experimental setups. Assembly and genome binning programs performed well for species represented by individual genomes but were substantially affected by the presence of related strains. Taxonomic profiling and binning programs were proficient at high taxonomic ranks, with a notable performance decrease below family level. Parameter settings markedly affected performance, underscoring their importance for program reproducibility. The CAMI results highlight current challenges but also provide a roadmap for software selection to answer specific research questions.
Original language | English |
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Pages (from-to) | 1063-1071 |
Number of pages | 9 |
Journal | NATURE METHODS |
Volume | 14 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2017 |
Externally published | Yes |
Bibliographical note
This work is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Keywords
- Metagenomics
- Classification and taxonomy
- Computational biology and bioinformatics
- Critical Assessment
- Metagenome Interpretation
- metagenomics software
- Critical Assessment of Metagenome Interpretation
- CAMI