Cryopreservation of sperm in farmed blacklip abalone (Haliotis rubra Leach, 1814)

Yi Liu, T Xu, Nicholas Robinson, Jianguang Qin, Xiaoxu Li

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    This study developed a technique of sperm cryopreservation using liquid nitrogen (LN) vapour in farmed blacklip abalone Haliotis rubra through evaluating the following five key factors: (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature; (3) thawing temperature; (4) sperm to egg ratio and (5) sugar addition, using sperm motility or fertilization rate as quality assessment indicators. The results demonstrated that 6% dimethyl sulfoxide (DMSO) was the best single CPA for sperm cryopreservation in this species. The highest post-thaw sperm motility was achieved when sperm were exposed to LN vapour for 10min at 5.2cm above the LN surface and thawed at 60°C and recovered at 16°C in seawater baths. Post-thaw sperm motility was found to be significantly higher when 6% DMSO was used in combination with 1% or 2% glucose than 6% DMSO alone. Further evaluation of fertilization rate between these CPAs showed that 6% DMSO+2% glucose achieved the highest fertilization rate of 70% at a sperm to egg ratio of 10000:1.

    Original languageEnglish
    Pages (from-to)2628-2636
    Number of pages9
    JournalAquaculture Research
    Volume46
    Issue number11
    Early online date2015
    DOIs
    Publication statusPublished - 1 Nov 2015

    Keywords

    • Farmed blacklip abalone
    • Glucose
    • Haliotis rubra
    • Sperm cryopreservation

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