Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

Gianny Scoleri; Jocelyn Choo; Lex Leong; Thomas Goddard; Lisa Shephard; Lucy Burr; Ivan Bastian; Rachel Thomson; Geraint Rogers

doi:10.1128/JCM.01410-16

Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

Gianny Scoleri, Jocelyn Choo, Lex Leong, Thomas Goddard, Lisa Shephard, Lucy Burr, Ivan Bastian, Rachel Thomson, Geraint Rogers

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

Original language	English
Pages (from-to)	2395-2398
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	54
Issue number	9
DOIs	https://doi.org/10.1128/JCM.01410-16
Publication status	Published - Sept 2016

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title = "Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples",

abstract = "Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.",

author = "Gianny Scoleri and Jocelyn Choo and Lex Leong and Thomas Goddard and Lisa Shephard and Lucy Burr and Ivan Bastian and Rachel Thomson and Geraint Rogers",

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AU - Scoleri, Gianny

AU - Choo, Jocelyn

AU - Leong, Lex

AU - Goddard, Thomas

AU - Shephard, Lisa

AU - Burr, Lucy

AU - Bastian, Ivan

AU - Thomson, Rachel

AU - Rogers, Geraint

PY - 2016/9

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AB - Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

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DO - 10.1128/JCM.01410-16

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SP - 2395

EP - 2398

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

Abstract

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