Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Obulisamy Parthiba Karthikeyan; Nadarajan Saravanan; Samuel Cirés; Carlos Alvarez-Roa; Ali Razaghi; Karthigeyan Chidambarampadmavathy; Chinnathambi Velu; Gobalakrishnan Subashchandrabose; Kirsten Ruth Heimann

doi:10.1080/09593330.2016.1198424

Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Obulisamy Parthiba Karthikeyan, Nadarajan Saravanan, Samuel Cirés, Carlos Alvarez-Roa, Ali Razaghi, Karthigeyan Chidambarampadmavathy, Chinnathambi Velu, Gobalakrishnan Subashchandrabose, Kirsten Ruth Heimann

Flinders University

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

Robust methanotrophic consortia for methane (CH₄) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH₄removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH₄under a very high gas flow rate (2.5 L min⁻¹; 20–25% CH₄) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C₁₆and C₁₈). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH₄in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min⁻¹; 1% CH₄). Contamination by cyanobacteria had beneficial effects on treating low-level CH₄, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH₄and CO₂at industrial scale.

Original language	English
Pages (from-to)	474-482
Number of pages	9
Journal	ENVIRONMENTAL TECHNOLOGY
Volume	38
Issue number	4
DOIs	https://doi.org/10.1080/09593330.2016.1198424
Publication status	Published - 2017

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Karthikeyan, O. P., Saravanan, N., Cirés, S., Alvarez-Roa, C., Razaghi, A., Chidambarampadmavathy, K., Velu, C., Subashchandrabose, G., & Heimann, K. R. (2017). Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters. ENVIRONMENTAL TECHNOLOGY, 38(4), 474-482. https://doi.org/10.1080/09593330.2016.1198424

Karthikeyan, Obulisamy Parthiba ; Saravanan, Nadarajan ; Cirés, Samuel ; Alvarez-Roa, Carlos ; Razaghi, Ali ; Chidambarampadmavathy, Karthigeyan ; Velu, Chinnathambi ; Subashchandrabose, Gobalakrishnan ; Heimann, Kirsten Ruth. / Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters. In: ENVIRONMENTAL TECHNOLOGY. 2017 ; Vol. 38, No. 4. pp. 474-482.

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abstract = "Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH4under a very high gas flow rate (2.5 L min−1; 20–25% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.",

author = "Karthikeyan, {Obulisamy Parthiba} and Nadarajan Saravanan and Samuel Cir{\'e}s and Carlos Alvarez-Roa and Ali Razaghi and Karthigeyan Chidambarampadmavathy and Chinnathambi Velu and Gobalakrishnan Subashchandrabose and Heimann, {Kirsten Ruth}",

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Karthikeyan, OP, Saravanan, N, Cirés, S, Alvarez-Roa, C, Razaghi, A, Chidambarampadmavathy, K, Velu, C, Subashchandrabose, G & Heimann, KR 2017, 'Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters', ENVIRONMENTAL TECHNOLOGY, vol. 38, no. 4, pp. 474-482. https://doi.org/10.1080/09593330.2016.1198424

Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters. / Karthikeyan, Obulisamy Parthiba; Saravanan, Nadarajan; Cirés, Samuel; Alvarez-Roa, Carlos; Razaghi, Ali; Chidambarampadmavathy, Karthigeyan; Velu, Chinnathambi; Subashchandrabose, Gobalakrishnan; Heimann, Kirsten Ruth.

In: ENVIRONMENTAL TECHNOLOGY, Vol. 38, No. 4, 2017, p. 474-482.

Research output: Contribution to journal › Article

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T1 - Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

AU - Karthikeyan, Obulisamy Parthiba

AU - Saravanan, Nadarajan

AU - Cirés, Samuel

AU - Alvarez-Roa, Carlos

AU - Razaghi, Ali

AU - Chidambarampadmavathy, Karthigeyan

AU - Velu, Chinnathambi

AU - Subashchandrabose, Gobalakrishnan

AU - Heimann, Kirsten Ruth

PY - 2017

Y1 - 2017

N2 - Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH4under a very high gas flow rate (2.5 L min−1; 20–25% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.

AB - Robust methanotrophic consortia for methane (CH4) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH4removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7–13% CH4under a very high gas flow rate (2.5 L min−1; 20–25% CH4) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C16and C18). Cultivation of the MMC on plastic bio-balls abated ∼95–97% CH4in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min−1; 1% CH4). Contamination by cyanobacteria had beneficial effects on treating low-level CH4, by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH4and CO2at industrial scale.

U2 - 10.1080/09593330.2016.1198424

DO - 10.1080/09593330.2016.1198424

M3 - Article

VL - 38

SP - 474

EP - 482

JO - ENVIRONMENTAL TECHNOLOGY

JF - ENVIRONMENTAL TECHNOLOGY

SN - 0959-3330

IS - 4

ER -