"Cycliplex PCR" confirmation of Fusobacterium necrophorum isolates from captive wallabies: A rapid and accurate approach

John Antiabong; Wayne Boardman; Ian Smith; Melissa Brown; Andrew Ball; Amanda Goodman

doi:10.1016/j.anaerobe.2012.12.003

"Cycliplex PCR" confirmation of Fusobacterium necrophorum isolates from captive wallabies: A rapid and accurate approach

John Antiabong, Wayne Boardman, Ian Smith, Melissa Brown, Andrew Ball, Amanda Goodman

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Isolation and identification of obligate anaerobic bacteria is labour intensive and time consuming. This has led to the increased application of molecular tools to circumvent part of this problem. We report here the development of a rapid, accurate and cost-effective method to isolate and identify Fusobacterium necrophorum species from South Australian wallaby populations using a supplemented medium (BHIRS) in conjunction with a " Cycliplex PCR" method which involves a stepwise-selective amplification of target PCR products. This report demonstrates the complementation of phenotypic characterization by PCR for accurate and fast identification of F. necrophorum isolates from wildlife origin.

Original language	English
Pages (from-to)	44-49
Number of pages	6
Journal	Anaerobe
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/j.anaerobe.2012.12.003
Publication status	Published - Feb 2013

Keywords

Captive wallaby
Cycliplex PCR
Fusobacterium necrophorum
Indole reaction
Isolation and identification
Supplemented medium
Wildlife

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.anaerobe.2012.12.003

Cite this

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abstract = "Isolation and identification of obligate anaerobic bacteria is labour intensive and time consuming. This has led to the increased application of molecular tools to circumvent part of this problem. We report here the development of a rapid, accurate and cost-effective method to isolate and identify Fusobacterium necrophorum species from South Australian wallaby populations using a supplemented medium (BHIRS) in conjunction with a {"} Cycliplex PCR{"} method which involves a stepwise-selective amplification of target PCR products. This report demonstrates the complementation of phenotypic characterization by PCR for accurate and fast identification of F. necrophorum isolates from wildlife origin.",

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author = "John Antiabong and Wayne Boardman and Ian Smith and Melissa Brown and Andrew Ball and Amanda Goodman",

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AU - Antiabong, John

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AU - Smith, Ian

AU - Brown, Melissa

AU - Ball, Andrew

AU - Goodman, Amanda

PY - 2013/2

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AB - Isolation and identification of obligate anaerobic bacteria is labour intensive and time consuming. This has led to the increased application of molecular tools to circumvent part of this problem. We report here the development of a rapid, accurate and cost-effective method to isolate and identify Fusobacterium necrophorum species from South Australian wallaby populations using a supplemented medium (BHIRS) in conjunction with a " Cycliplex PCR" method which involves a stepwise-selective amplification of target PCR products. This report demonstrates the complementation of phenotypic characterization by PCR for accurate and fast identification of F. necrophorum isolates from wildlife origin.

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KW - Cycliplex PCR

KW - Fusobacterium necrophorum

KW - Indole reaction

KW - Isolation and identification

KW - Supplemented medium

KW - Wildlife

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M3 - Article

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JO - Anaerobe

JF - Anaerobe

IS - 1

ER -

"Cycliplex PCR" confirmation of Fusobacterium necrophorum isolates from captive wallabies: A rapid and accurate approach

Abstract

Keywords

UN SDGs

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