"Cycliplex PCR" confirmation of Fusobacterium necrophorum isolates from captive wallabies: A rapid and accurate approach

John Antiabong, Wayne Boardman, Ian Smith, Melissa Brown, Andrew Ball, Amanda Goodman

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Isolation and identification of obligate anaerobic bacteria is labour intensive and time consuming. This has led to the increased application of molecular tools to circumvent part of this problem. We report here the development of a rapid, accurate and cost-effective method to isolate and identify Fusobacterium necrophorum species from South Australian wallaby populations using a supplemented medium (BHIRS) in conjunction with a " Cycliplex PCR" method which involves a stepwise-selective amplification of target PCR products. This report demonstrates the complementation of phenotypic characterization by PCR for accurate and fast identification of F. necrophorum isolates from wildlife origin.

    Original languageEnglish
    Pages (from-to)44-49
    Number of pages6
    JournalAnaerobe
    Volume19
    Issue number1
    DOIs
    Publication statusPublished - Feb 2013

    Keywords

    • Captive wallaby
    • Cycliplex PCR
    • Fusobacterium necrophorum
    • Indole reaction
    • Isolation and identification
    • Supplemented medium
    • Wildlife

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