Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

Peter J. Adams; Paul T. Monis; Aileen D. Elliot; R. C.Andrew Thompson

doi:10.1016/j.meegid.2004.05.003

Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

Peter J. Adams
, Paul T. Monis
, Aileen D. Elliot
, R. C.Andrew Thompson

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.

Original language	English
Pages (from-to)	365-370
Number of pages	6
Journal	Infection, Genetics and Evolution
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.meegid.2004.05.003
Publication status	Published - Dec 2004
Externally published	Yes

Keywords

Ef1α
Giardia
Marsupial
Phylogenetic
Small subunit rDNA

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title = "Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia",

abstract = "Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.",

keywords = "Ef1α, Giardia, Marsupial, Phylogenetic, Small subunit rDNA",

author = "Adams, \{Peter J.\} and Monis, \{Paul T.\} and Elliot, \{Aileen D.\} and Thompson, \{R. C.Andrew\}",

year = "2004",

month = dec,

doi = "10.1016/j.meegid.2004.05.003",

language = "English",

volume = "4",

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journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

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TY - JOUR

T1 - Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

AU - Adams, Peter J.

AU - Monis, Paul T.

AU - Elliot, Aileen D.

AU - Thompson, R. C.Andrew

PY - 2004/12

Y1 - 2004/12

N2 - Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.

AB - Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.

KW - Ef1α

KW - Giardia

KW - Marsupial

KW - Phylogenetic

KW - Small subunit rDNA

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U2 - 10.1016/j.meegid.2004.05.003

DO - 10.1016/j.meegid.2004.05.003

M3 - Article

C2 - 15374535

AN - SCOPUS:4544290936

SN - 1567-1348

VL - 4

SP - 365

EP - 370

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

IS - 4

ER -

Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

Abstract

Keywords

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