Delineation of the IGF-II C domain elements involved in binding and activation of the IR-A, IR-B and IGF-IR

Stephen Henderson, GV Brierley, Katharina Surinya, Ilka Priebe, David Catcheside, John Wallace, Briony Forbes, L J Cosgrove

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Objective: Human insulin-like growth factor-I and -II (IGF-I and -II) ligands share a high degree of sequence and structural homology. Despite their similarities, IGF-I and IGF-II exhibit differential receptor binding and activation characteristics. The C domains of IGF-I and IGF-II are the primary determinants of binding specificity to the insulin-like growth factor I receptor (IGF-IR), insulin receptor exon 11- (IR-A) and exon 11. + (IR-B) isoforms. Design: Three IGF-II analogues were generated in order to delineate the C domain residues that confer the differential receptor binding affinity and activation properties of the IGFs. Chimeric IGF-II analogues IGF-IICIN and IGF-IICIC contained partial IGF-I C domain substitutions (IGF-I residues underlined) GYGSSSRRSR and SRVSRRAPQT, respectively. Results: The IGF-IICIN analogue bound the IR-A and IGF-IR with high affinity but bound the IR-B with a relatively lower affinity than IGF-II, suggesting a negative interaction between the exon-11 encoded peptide in the IR-B and the C-domain. The ability of IGF-IICIN to activate receptors and elicit cell viability responses was generally proportional to its relative receptor binding affinity but appeared to act as a partial agonist equivalent to IGF-I when binding and activating the IGF-IR. In contrast, IGF-IICIC bound IGF-IR with high affinity but elicited lower receptor activation and cell viability responses. Analogue IGF-IICIS contained a truncated IGF-I C domain (GSSSRRAT) and generally displayed a relatively poor ability to bind, activate and elicit viability responses via each receptor. Conclusions: Together, the IGF analogues demonstrate that both flanks of the IGF-II C domain play important roles in the greater ability of IGF-II to bind and activate IR receptors than IGF-I.

    Original languageEnglish
    Pages (from-to)20-27
    Number of pages8
    JournalGrowth Hormone and Igf Research
    Volume25
    Issue number1
    DOIs
    Publication statusPublished - 1 Feb 2015

    Keywords

    • Binding
    • Chimera
    • IGF-I
    • IGF-II
    • Insulin receptor
    • Structure

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