Destruction of locus coeruleus neuronal perikarya after injection of anti-dopamine-B-hydroxylase immunotoxin into the olfactory bulb of the rat

W. W. Blessing, D. A. Lappi, R. G. Wiley

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    33 Citations (Scopus)

    Abstract

    Saporin, a ribosome-inactivating protein, was coupled to a monoclonal antibody to dopamine-B-hydroxylase (DBH) and injected unilaterally into the olfactory bulb of rats. After 4-13 days survival, the rat brain was processed histologically and the locus coerulei (LC) examined with Nissl and anti-DBH staining. There were degenerating dendrites in surviving LC neurons on the side ipsilateral to the immunotoxin-injected olfactory bulb. The number of Nissl-positive LC neurons in a transverse section through the caudal one third of the LC was reduced from 116 ± 10 to 50 ± 8 neurons (P < 0.01, n = 7) and the number of DBH-positive neurons in the more rostral LC sections was reduced from 13 ± 2 to 5 ± 1 (P < 0.05, n = 4). Our results indicate that it is possible to lesion LC neurons via retrograde intraaxonal transport of saporin-anti-DBH immunotoxin from the olfactory bulb.

    Original languageEnglish
    Pages (from-to)85-88
    Number of pages4
    JournalNeuroscience letters
    Volume243
    Issue number1-3
    DOIs
    Publication statusPublished - 27 Feb 1998

    Keywords

    • Locus coeruleus
    • Neurotoxin
    • Noradrenaline
    • Olfactory bulb
    • Retrograde transport
    • Ribosomes

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