Detection of chlamydia trachomatis with fluorescent monoclonal antibody

Peter G. Hallsworth, P. J. McDonald

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Commercial kits of fluorescein-labelled monoclonal antibody against Chlamydia trachomatis have been evaluated (1) as an alternative to Giemsa staining for detection of chlamydia inclusions in cell culture and (2) for direct detection of chlamydia in conjunctival, urethral and cervical smears. The inclusion detection kit (MicroTrak Culture Confirmation Test) was tested on 270 cultures and was found to be highly sensitive, detecting all 16 Giemsa-positive specimens plus an additional 3 that were negative by Giemsa. It was also superior to Giemsa-staining in terms of simplicity of use, ease of detection, and readability with toxic specimens. The direct detection kit (MicroTrak Direct Specimen Test) gave results which agreed with the culture result for all 33 genital tract specimens and for 32 of 34 conjunctival specimens tested in parallel. The kit is considered to be a valuable test for diagnosis of chlamydial infection by laboratories lacking adequate tissue-culture facilities.

Original languageEnglish
Pages (from-to)500-502
Number of pages3
JournalPathology
Volume17
Issue number3
DOIs
Publication statusPublished - 25 Jan 1985
Externally publishedYes

Keywords

  • Cell-culture inclusions
  • Chlamydia trachomatis
  • Direct detection
  • Monoclonal antibody

Fingerprint Dive into the research topics of 'Detection of chlamydia trachomatis with fluorescent monoclonal antibody'. Together they form a unique fingerprint.

  • Cite this