Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Karen Bruce, Amanda Ellis, Sophie Leterme, Dmitriy Khodakov, Claire Lenehan

    Research output: Contribution to conferencePaper

    Abstract

    Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

    Original languageEnglish
    DOIs
    Publication statusPublished - 1 Dec 2013
    EventMicro/Nano Materials, Devices, and Systems -
    Duration: 8 Dec 2013 → …

    Conference

    ConferenceMicro/Nano Materials, Devices, and Systems
    Period8/12/13 → …

    Keywords

    • Alexandrium catenella
    • covalent attachment
    • Harmful algal bloom (HAB)
    • hybridisation
    • surface modification

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