Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Karen Bruce; Amanda Ellis; Sophie Leterme; Dmitriy Khodakov; Claire Lenehan

doi:10.1117/12.2034011

Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Karen Bruce, Amanda Ellis, Sophie Leterme, Dmitriy Khodakov, Claire Lenehan

Research output: Contribution to conference › Paper › peer-review

Abstract

Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

Original language	English
DOIs	https://doi.org/10.1117/12.2034011
Publication status	Published - 1 Dec 2013
Event	Micro/Nano Materials, Devices, and Systems - Duration: 8 Dec 2013 → …

Conference

Conference	Micro/Nano Materials, Devices, and Systems
Period	8/12/13 → …

Keywords

Alexandrium catenella
covalent attachment
Harmful algal bloom (HAB)
hybridisation
surface modification

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10.1117/12.2034011

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title = "Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces",

abstract = "Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.",

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AU - Ellis, Amanda

AU - Leterme, Sophie

AU - Khodakov, Dmitriy

AU - Lenehan, Claire

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

AB - Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

KW - Alexandrium catenella

KW - covalent attachment

KW - Harmful algal bloom (HAB)

KW - hybridisation

KW - surface modification

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DO - 10.1117/12.2034011

M3 - Paper

T2 - Micro/Nano Materials, Devices, and Systems

Y2 - 8 December 2013

ER -

Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Abstract

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Keywords

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