This study investigates the possibility of using spectral analysis of the closing sounds of a prosthetic heart valve to indicate minor changes to its mechanical state. Sounds are recorded in vitro in a large effectively anechoic tank of water, and the elastically mounted valve is actuated pneumatically within the water. This provides a noise-free environment for recordings. Analysis of the valve sounds is by spectral averaging and digital filtering, with each individual valve used to create its own filter. Results show that it is possible using this technique to detect reliably changes in the mechanical state of a valve, such as the addition of a small mass to the structure of the valve.