Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Leung; Feng Guo; Yuning Hong; Engui Zhao; Ryan Kwok; Nelson Leung; Sijie Chen; Nishant Vaikath; Omar El-Agnaf; Youhong Tang; Wei-Ping Gai; Ben Tang

doi:10.1039/c4cc07911f

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Leung, Feng Guo, Yuning Hong, Engui Zhao, Ryan Kwok, Nelson Leung, Sijie Chen, Nishant Vaikath, Omar El-Agnaf, Youhong Tang, Wei-Ping Gai, Ben Tang

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74 Citations (Scopus)

Abstract

We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

Original language	English
Pages (from-to)	1866-1869
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	10
DOIs	https://doi.org/10.1039/c4cc07911f
Publication status	Published - 4 Feb 2015

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title = "Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence",

abstract = "We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.",

author = "Chris Leung and Feng Guo and Yuning Hong and Engui Zhao and Ryan Kwok and Nelson Leung and Sijie Chen and Nishant Vaikath and Omar El-Agnaf and Youhong Tang and Wei-Ping Gai and Ben Tang",

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T1 - Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

AU - Leung, Chris

AU - Guo, Feng

AU - Hong, Yuning

AU - Zhao, Engui

AU - Kwok, Ryan

AU - Leung, Nelson

AU - Chen, Sijie

AU - Vaikath, Nishant

AU - El-Agnaf, Omar

AU - Tang, Youhong

AU - Gai, Wei-Ping

AU - Tang, Ben

PY - 2015/2/4

Y1 - 2015/2/4

N2 - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

AB - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

UR - http://www.scopus.com/inward/record.url?scp=84921506541&partnerID=8YFLogxK

U2 - 10.1039/c4cc07911f

DO - 10.1039/c4cc07911f

M3 - Article

SN - 1359-7345

VL - 51

SP - 1866

EP - 1869

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

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