Detection of Toxoplasma-specific igM in cord blood sera by antibody-class capture enzyme-linked immunosorbent assay

Alan M. Johnson, H. Roberts, P. J. McDonald, J. Rothe

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    An antibody class capture (ACC) enzyme-linked immunosorbent assay (ELISA) utilizing an F(ab)2 conjugate was established and its specificity and reproducibility were determined. The test was further validated by comparing its results with those obtained by 2 overseas reference laboratories which used the indirect immunofluorescence antibody test (IIFT) for Toxoplasma-specific IgM. It was found that the F(ab)2ACC-ELISA was not affected by the presence of rheumatoid and antinuclear factor in serum, that it was reproducible (coefficients of variation less than 10.0%), and that it compared well with tests currently used overseas. ELISA was then used to test for the presence of Toxoplasma-specific IgM in cord blood sera. The results obtained to date are consistent with the hypothesis that the prevalence of congenital toxoplasmosis in the population of Adelaide is less than 1/2, 000.

    Original languageEnglish
    Pages (from-to)586-589
    Number of pages4
    JournalPathology
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - Oct 1985

    Keywords

    • Antibody class capture
    • Cord blood
    • Enzyme-linked immunosorbent assay
    • IgM
    • Toxoplasmosis

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