TY - JOUR
T1 - Determinants of UDP glucuronosyltransferase membrane association and residency in the endoplasmic reticulum
AU - Meech, R.
AU - MacKenzie, P. I.
PY - 1998/8/1
Y1 - 1998/8/1
N2 - The UDP glucuronosyltransferases (UGT) are a family of enzymes which detoxify small hydrophobic compounds in mammalian cells. It is believed that UGTs are type I endoplasmic reticulum (ER) resident membrane proteins with a single membrane spanning domain near the carboxyl-terminus. The determinants of endoplasmic reticulum subcellular localization and membrane association for the UDP glucuronosyltransferase, UGT2B1, were examined. The construction and analysis of truncated and chimeric forms of UGT2B1 demonstrated that the protein contains regions of membrane interaction in the amino-terminal half of the lumenal domain in addition to the carboxyl-terminal transmembrane domain. UGT2B1 also remained resident in the ER in the absence of the cytosolic tail and transmembrane domain. Construction and analysis of an active, truncated form of UGT2B1 indicated that the cytosolically located dilysine motif, which is a putative ER membrane targeting signal, may be redundant for residency of UGT in the ER.
AB - The UDP glucuronosyltransferases (UGT) are a family of enzymes which detoxify small hydrophobic compounds in mammalian cells. It is believed that UGTs are type I endoplasmic reticulum (ER) resident membrane proteins with a single membrane spanning domain near the carboxyl-terminus. The determinants of endoplasmic reticulum subcellular localization and membrane association for the UDP glucuronosyltransferase, UGT2B1, were examined. The construction and analysis of truncated and chimeric forms of UGT2B1 demonstrated that the protein contains regions of membrane interaction in the amino-terminal half of the lumenal domain in addition to the carboxyl-terminal transmembrane domain. UGT2B1 also remained resident in the ER in the absence of the cytosolic tail and transmembrane domain. Construction and analysis of an active, truncated form of UGT2B1 indicated that the cytosolically located dilysine motif, which is a putative ER membrane targeting signal, may be redundant for residency of UGT in the ER.
KW - ER residency
KW - Membrane association
KW - UDP glucuronosyltransferase
UR - http://www.scopus.com/inward/record.url?scp=0032147023&partnerID=8YFLogxK
U2 - 10.1006/abbi.1998.0750
DO - 10.1006/abbi.1998.0750
M3 - Article
C2 - 9681994
AN - SCOPUS:0032147023
VL - 356
SP - 77
EP - 85
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -