Determination of NG-nitro-l-arginine and NG-nitro-l-arginine methyl ester in plasma by high-performance liquid chromatography

M. J. Whiting, A. J. Rutten, P. Williams, A. D. Bersten

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An HPLC method has been developed for the measurement of the nitric oxide synthase inhibitors, NG-nitro-l-arginine (l-NOLA) and NG-nitro-l-arginine methyl ester (l-NAME), in sheep plasma. Using an ion-exchange HPLC column (JWAS 150, 100 × 3.9 mm I.D., Millipore-Waters, Australia) and post-column ninhydrin detection, l-NOLA was separated from valine and other plasma amino acids. When added to sheep plasma, good recovery (mean 102%) and precision (mean coefficient of variation 2.7%) in the measurement of l-NOLA was obtained over the range 2-50 mg/l. l-NAME was unstable in sheep plasma at 37°C, and was converted to l-NOLA with a half-life of 250 min. This method will permit pharmacokinetic parameters to be determined for these potential drugs, and will allow plasma drug concentrations to be correlated with the pharmacodynamic effects of these compounds.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume660
Issue number1
DOIs
Publication statusPublished - 3 Oct 1994
Externally publishedYes

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