TY - JOUR
T1 - Development of 18 microsatellite markers for the southern purple-spotted gudgeon (Mogurnda adspersa) from the lower Murray-Darling Basin through 454 pyrosequencing
AU - de Carvalho, Daniel
AU - Sasaki, Minami
AU - Hammer, Michael
AU - Beheregaray, Luciano
PY - 2012
Y1 - 2012
N2 - A new set of 18 microsatellite loci was developed for the threatened Australian freshwater fish southern purple-spotted gudgeon Mogurnda adspersa (Eleotridae) using a next generation sequencing approach. A total of 84 fish from two populations (including one rescued into captivity) were successfully genotyped at all markers using a multiplex approach. As expected for threatened species, we observed relatively low genetic variation across most loci (average allelic diversity = 5.4; average heterozygosity = 0.380). No evidence for linkage disequilibrium was detected and all loci were in Hardy-Weinberg equilibrium. This new set of microsatellite markers will benefit substantially the ongoing conservation program of a critically endangered population of M. adspersa that involves captive breeding, relatedness and paternity analyses, reintroduction, and landscape genetics.
AB - A new set of 18 microsatellite loci was developed for the threatened Australian freshwater fish southern purple-spotted gudgeon Mogurnda adspersa (Eleotridae) using a next generation sequencing approach. A total of 84 fish from two populations (including one rescued into captivity) were successfully genotyped at all markers using a multiplex approach. As expected for threatened species, we observed relatively low genetic variation across most loci (average allelic diversity = 5.4; average heterozygosity = 0.380). No evidence for linkage disequilibrium was detected and all loci were in Hardy-Weinberg equilibrium. This new set of microsatellite markers will benefit substantially the ongoing conservation program of a critically endangered population of M. adspersa that involves captive breeding, relatedness and paternity analyses, reintroduction, and landscape genetics.
KW - Captive breeding
KW - Conservation program
KW - Critically endangered population
KW - Evolutionary significant units
KW - Reintroduction
KW - Relatedness
UR - http://www.scopus.com/inward/record.url?scp=84883734464&partnerID=8YFLogxK
U2 - 10.1007/s12686-011-9542-0
DO - 10.1007/s12686-011-9542-0
M3 - Article
VL - 4
SP - 339
EP - 341
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 2
ER -