Development of 18 microsatellite markers for the southern purple-spotted gudgeon (Mogurnda adspersa) from the lower Murray-Darling Basin through 454 pyrosequencing

Daniel de Carvalho, Minami Sasaki, Michael Hammer, Luciano Beheregaray

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    5 Citations (Scopus)

    Abstract

    A new set of 18 microsatellite loci was developed for the threatened Australian freshwater fish southern purple-spotted gudgeon Mogurnda adspersa (Eleotridae) using a next generation sequencing approach. A total of 84 fish from two populations (including one rescued into captivity) were successfully genotyped at all markers using a multiplex approach. As expected for threatened species, we observed relatively low genetic variation across most loci (average allelic diversity = 5.4; average heterozygosity = 0.380). No evidence for linkage disequilibrium was detected and all loci were in Hardy-Weinberg equilibrium. This new set of microsatellite markers will benefit substantially the ongoing conservation program of a critically endangered population of M. adspersa that involves captive breeding, relatedness and paternity analyses, reintroduction, and landscape genetics.

    Original languageEnglish
    Pages (from-to)339-341
    Number of pages3
    JournalConservation Genetics Resources
    Volume4
    Issue number2
    DOIs
    Publication statusPublished - 2012

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