Development of 21 microsatellite markers for the threatened Yarra pygmy perch (Nannoperca obscura) through 454 shot-gun pyrosequencing

Daniel de Carvalho, Clara Rodriguez Zarate, Michael Hammer, Luciano Beheregaray

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Using a next generation sequencing approach, a set of 21 new microsatellites loci was developed from the threatened freshwater fish Yarra pygmy perch (YPP) Nannoperca obscura (Percichthyidae). All markers were successfully genotyped using 28 YPP kept in captivity. These animals represent an entire population rescued from the lower Murray-Darling Basin in Australia before its habitat dried out. As expected due to the critical conservation status of the population, we observed low genetic variation across most loci (mean number of alleles per locus = 2.76; mean heterozygosity = 0.28). No deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were detected and only one locus (Nob30) showed evidence for null alleles. These molecular markers represent important resources for the ongoing YPP captive breeding program, and for upcoming restoration and landscape genetic studies of this species.

    Original languageEnglish
    Pages (from-to)601-604
    Number of pages4
    JournalConservation Genetics Resources
    Volume3
    Issue number4
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • Freshwater fish
    • Microsatellite development
    • Nannoperca obscura
    • Next Generation DNA sequencing

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