Development of a highly sensitive assay, based on the polymerase chain reaction, for rare B‐lymphocyte clones in a polyclonal population

M. J. Brisco, L. W. Tan, A. M. Orsborn, A. A. Morley

    Research output: Contribution to journalArticle

    204 Citations (Scopus)

    Abstract

    A method has been developed to use the polymerase chain reaction to amplify and sequence the chain determining region 3 (CDR3) of the human immunoglobulin heavy‐chain gene, and to use the sequence as a marker for rare neoplastic B lymphocytes. Consensus primers for the Variable and Joining regions of the gene were constructed and shown to enable efficient amplification, directed cloning, and sequencing of CDR3. Using leukaemic cell line PFMC as a test system, CDR3 was sequenced, specific primers synthesized, and PFMC DNA was detected down to a dilution of 1:1300 in DNA from normal lymphocytes. This strategy should be useful for monitoring therapy and detecting early disease relapse in B lymphoproliferative disease.

    Original languageEnglish
    Pages (from-to)163-167
    Number of pages5
    JournalBritish Journal of Haematology
    Volume75
    Issue number2
    DOIs
    Publication statusPublished - Jun 1990

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