Development of a programmable freezing technique on larval cryopreservation in the Pacific oyster Crassostrea gigas

Yibing Liu; Mark Gluis; Penny Miller-Ezzy; Jiabo Han; Jianguang Qin; Xin Zhan; Xiaoxu Li

doi:10.1016/j.aquaculture.2020.735199

Development of a programmable freezing technique on larval cryopreservation in the Pacific oyster Crassostrea gigas

Yibing Liu, Mark Gluis, Penny Miller-Ezzy, Jiabo Han, Jianguang Qin, Xin Zhan, Xiaoxu Li

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage of larvae that develop into D-larvae after 24 h post-fertilization. The results showed that the post-thaw D-larval rate above 80% was achieved when the 18 h post-fertilization (PF) larvae were cryopreserved with the CPA consisting of 10% ethylene glycol +5% Ficoll +0.2% polyvinylpyrrolidone. No significant difference in the rate of post-thaw D-larvae (P > .05) was found between initial larval density of 4 × 10⁵ mL⁻¹ and 1 × 10⁶ mL⁻¹. The shell length of progenies produced from fresh and cryopreserved larvae had no significant difference at all developmental stages investigated. In addition, similar relative mortality rates were found from umbo larvae (day 8 PF) to spat (day 27 PF) between progenies produced from fresh and cryopreserved larvae. Therefore, the larval cryopreservation technique developed in this study could be used to facilitate breeding programs and hatchery management in Pacific oyster aquaculture industry.

Original language	English
Article number	735199
Number of pages	4
Journal	Aquaculture
Volume	523
DOIs	https://doi.org/10.1016/j.aquaculture.2020.735199
Publication status	Published - 30 Jun 2020

Bibliographical note

Funding Information:
This research was funded by the Talent Project of Revitalizing Liaoning (Project No. XLYC1807087 ), Department of Ocean and Fisheries of Liaoning (Project No. 201829 ), China Scholarship Council and South Australian Research and Development Institute . We thank Mr. Gary Zippel of Zippel Enterprises Pty Ltd. for the provision of Pacific oyster broodstock.

Publisher Copyright:
© 2020

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Crassostrea gigas
Ficoll
Larval cryopreservation
Polyvinylpyrrolidone

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10.1016/j.aquaculture.2020.735199Licence: In Copyright

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title = "Development of a programmable freezing technique on larval cryopreservation in the Pacific oyster Crassostrea gigas",

abstract = "This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage of larvae that develop into D-larvae after 24 h post-fertilization. The results showed that the post-thaw D-larval rate above 80% was achieved when the 18 h post-fertilization (PF) larvae were cryopreserved with the CPA consisting of 10% ethylene glycol +5% Ficoll +0.2% polyvinylpyrrolidone. No significant difference in the rate of post-thaw D-larvae (P > .05) was found between initial larval density of 4 × 105 mL−1 and 1 × 106 mL−1. The shell length of progenies produced from fresh and cryopreserved larvae had no significant difference at all developmental stages investigated. In addition, similar relative mortality rates were found from umbo larvae (day 8 PF) to spat (day 27 PF) between progenies produced from fresh and cryopreserved larvae. Therefore, the larval cryopreservation technique developed in this study could be used to facilitate breeding programs and hatchery management in Pacific oyster aquaculture industry.",

keywords = "Crassostrea gigas, Ficoll, Larval cryopreservation, Polyvinylpyrrolidone",

author = "Yibing Liu and Mark Gluis and Penny Miller-Ezzy and Jiabo Han and Jianguang Qin and Xin Zhan and Xiaoxu Li",

note = "Funding Information: This research was funded by the Talent Project of Revitalizing Liaoning (Project No. XLYC1807087 ), Department of Ocean and Fisheries of Liaoning (Project No. 201829 ), China Scholarship Council and South Australian Research and Development Institute . We thank Mr. Gary Zippel of Zippel Enterprises Pty Ltd. for the provision of Pacific oyster broodstock. Publisher Copyright: {\textcopyright} 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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AU - Gluis, Mark

AU - Miller-Ezzy, Penny

AU - Han, Jiabo

AU - Qin, Jianguang

AU - Zhan, Xin

AU - Li, Xiaoxu

N1 - Funding Information: This research was funded by the Talent Project of Revitalizing Liaoning (Project No. XLYC1807087 ), Department of Ocean and Fisheries of Liaoning (Project No. 201829 ), China Scholarship Council and South Australian Research and Development Institute . We thank Mr. Gary Zippel of Zippel Enterprises Pty Ltd. for the provision of Pacific oyster broodstock. Publisher Copyright: © 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/6/30

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N2 - This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage of larvae that develop into D-larvae after 24 h post-fertilization. The results showed that the post-thaw D-larval rate above 80% was achieved when the 18 h post-fertilization (PF) larvae were cryopreserved with the CPA consisting of 10% ethylene glycol +5% Ficoll +0.2% polyvinylpyrrolidone. No significant difference in the rate of post-thaw D-larvae (P > .05) was found between initial larval density of 4 × 105 mL−1 and 1 × 106 mL−1. The shell length of progenies produced from fresh and cryopreserved larvae had no significant difference at all developmental stages investigated. In addition, similar relative mortality rates were found from umbo larvae (day 8 PF) to spat (day 27 PF) between progenies produced from fresh and cryopreserved larvae. Therefore, the larval cryopreservation technique developed in this study could be used to facilitate breeding programs and hatchery management in Pacific oyster aquaculture industry.

AB - This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage of larvae that develop into D-larvae after 24 h post-fertilization. The results showed that the post-thaw D-larval rate above 80% was achieved when the 18 h post-fertilization (PF) larvae were cryopreserved with the CPA consisting of 10% ethylene glycol +5% Ficoll +0.2% polyvinylpyrrolidone. No significant difference in the rate of post-thaw D-larvae (P > .05) was found between initial larval density of 4 × 105 mL−1 and 1 × 106 mL−1. The shell length of progenies produced from fresh and cryopreserved larvae had no significant difference at all developmental stages investigated. In addition, similar relative mortality rates were found from umbo larvae (day 8 PF) to spat (day 27 PF) between progenies produced from fresh and cryopreserved larvae. Therefore, the larval cryopreservation technique developed in this study could be used to facilitate breeding programs and hatchery management in Pacific oyster aquaculture industry.

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ER -

Development of a programmable freezing technique on larval cryopreservation in the Pacific oyster Crassostrea gigas

Abstract

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Keywords

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