Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

Juergen von Zum Hof, K Schonrogge, James Cook, Michael Gardner

    Research output: Contribution to journalArticle

    Abstract

    To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers. We describe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97.

    Original languageEnglish
    Pages (from-to)108-110
    Number of pages3
    JournalAustralian Journal of Zoology
    Volume60
    Issue number2
    DOIs
    Publication statusPublished - 2012

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