Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

James Herbert; Stephanie Godfrey; Christopher Bull; Robert Menz

doi:10.1016/j.ijpara.2010.03.018

Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

James Herbert, Stephanie Godfrey, Christopher Bull, Robert Menz

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The developmental stages of Hepatozoon tuatarae were elucidated in both the tuatara host, Sphenodon punctatus and the tick, Amblyomma sphenodonti. PCR amplicons from A. sphenodonti samples identified DNA matching H. tuatarae. Dissection of tick samples showed oogenesis and sporogony occurring in the haemocoel of A. sphenodonti with the average mature oocyst size being 236 × 228 μm. Partial sequence data of the parasite's small subunit ribosomal gene, obtained by PCR, was used for phylogenetic comparison. Characterisation of the H. tuatarae lifecycle will help in conservation management of the tuatara.

Original language	English
Pages (from-to)	1311-1315
Number of pages	5
Journal	International Journal For Parasitology
Volume	40
Issue number	11
DOIs	https://doi.org/10.1016/j.ijpara.2010.03.018
Publication status	Published - Sept 2010

Keywords

18S rRNA
Apicomplexa
Hepatozoon tuatarae
Ixodidae
Life cycle
Tuatara

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijpara.2010.03.018

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title = "Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti",

abstract = "The developmental stages of Hepatozoon tuatarae were elucidated in both the tuatara host, Sphenodon punctatus and the tick, Amblyomma sphenodonti. PCR amplicons from A. sphenodonti samples identified DNA matching H. tuatarae. Dissection of tick samples showed oogenesis and sporogony occurring in the haemocoel of A. sphenodonti with the average mature oocyst size being 236 × 228 μm. Partial sequence data of the parasite's small subunit ribosomal gene, obtained by PCR, was used for phylogenetic comparison. Characterisation of the H. tuatarae lifecycle will help in conservation management of the tuatara.",

keywords = "18S rRNA, Apicomplexa, Hepatozoon tuatarae, Ixodidae, Life cycle, Tuatara",

author = "James Herbert and Stephanie Godfrey and Christopher Bull and Robert Menz",

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doi = "10.1016/j.ijpara.2010.03.018",

language = "English",

volume = "40",

pages = "1311--1315",

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Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti. / Herbert, James; Godfrey, Stephanie; Bull, Christopher et al.
In: International Journal For Parasitology, Vol. 40, No. 11, 09.2010, p. 1311-1315.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

AU - Herbert, James

AU - Godfrey, Stephanie

AU - Bull, Christopher

AU - Menz, Robert

PY - 2010/9

Y1 - 2010/9

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AB - The developmental stages of Hepatozoon tuatarae were elucidated in both the tuatara host, Sphenodon punctatus and the tick, Amblyomma sphenodonti. PCR amplicons from A. sphenodonti samples identified DNA matching H. tuatarae. Dissection of tick samples showed oogenesis and sporogony occurring in the haemocoel of A. sphenodonti with the average mature oocyst size being 236 × 228 μm. Partial sequence data of the parasite's small subunit ribosomal gene, obtained by PCR, was used for phylogenetic comparison. Characterisation of the H. tuatarae lifecycle will help in conservation management of the tuatara.

KW - 18S rRNA

KW - Apicomplexa

KW - Hepatozoon tuatarae

KW - Ixodidae

KW - Life cycle

KW - Tuatara

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DO - 10.1016/j.ijpara.2010.03.018

M3 - Article

SN - 0020-7519

VL - 40

SP - 1311

EP - 1315

JO - International Journal For Parasitology

JF - International Journal For Parasitology

IS - 11

ER -

Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

Abstract

Keywords

UN SDGs

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