Developmental validation of STRmix™ NGS, a probabilistic genotyping tool for the interpretation of autosomal STRs from forensic profiles generated using NGS

Kevin Cheng; Jo-Anne Bright; Hannah Kelly; Yao Yuan Liu; Meng Han Lin; Maarten Kruijver; Duncan Taylor; John Buckleton

doi:10.1016/j.fsigen.2022.102804

Developmental validation of STRmix™ NGS, a probabilistic genotyping tool for the interpretation of autosomal STRs from forensic profiles generated using NGS

Kevin Cheng, Jo-Anne Bright, Hannah Kelly, Yao Yuan Liu, Meng Han Lin, Maarten Kruijver, Duncan Taylor, John Buckleton

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We describe the developmental validation of the probabilistic genotyping software – STRmix™ NGS – developed for the interpretation of forensic DNA profiles containing autosomal STRs generated using next generation sequencing (NGS) also known as massively parallel sequencing (MPS) technologies. Developmental validation was carried out in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM) Guidelines for the Validation of Probabilistic Genotyping Systems and the International Society for Forensic Genetics (ISFG) recommendations and included sensitivity and specificity testing, accuracy, precision, and the interpretation of case-types samples. The results of developmental validation demonstrate the appropriateness of the software for the interpretation of profiles developed using NGS technology.

Original language	English
Article number	102804
Number of pages	16
Journal	Forensic Science International: Genetics
Volume	62
Early online date	8 Nov 2022
DOIs	https://doi.org/10.1016/j.fsigen.2022.102804
Publication status	Published - Jan 2023
Externally published	Yes

Keywords

Developmental validation
MPS
NGS
Probabilistic genotyping
STRs

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10.1016/j.fsigen.2022.102804Licence: In Copyright

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Cheng, K., Bright, J.-A., Kelly, H., Liu, Y. Y., Lin, M. H., Kruijver, M., Taylor, D., & Buckleton, J. (2023). Developmental validation of STRmix™ NGS, a probabilistic genotyping tool for the interpretation of autosomal STRs from forensic profiles generated using NGS. Forensic Science International: Genetics, 62, Article 102804. https://doi.org/10.1016/j.fsigen.2022.102804

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abstract = "We describe the developmental validation of the probabilistic genotyping software – STRmix{\texttrademark} NGS – developed for the interpretation of forensic DNA profiles containing autosomal STRs generated using next generation sequencing (NGS) also known as massively parallel sequencing (MPS) technologies. Developmental validation was carried out in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM) Guidelines for the Validation of Probabilistic Genotyping Systems and the International Society for Forensic Genetics (ISFG) recommendations and included sensitivity and specificity testing, accuracy, precision, and the interpretation of case-types samples. The results of developmental validation demonstrate the appropriateness of the software for the interpretation of profiles developed using NGS technology.",

keywords = "Developmental validation, MPS, NGS, Probabilistic genotyping, STRs",

author = "Kevin Cheng and Jo-Anne Bright and Hannah Kelly and Liu, {Yao Yuan} and Lin, {Meng Han} and Maarten Kruijver and Duncan Taylor and John Buckleton",

year = "2023",

month = jan,

doi = "10.1016/j.fsigen.2022.102804",

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AU - Cheng, Kevin

AU - Bright, Jo-Anne

AU - Kelly, Hannah

AU - Liu, Yao Yuan

AU - Lin, Meng Han

AU - Kruijver, Maarten

AU - Taylor, Duncan

AU - Buckleton, John

PY - 2023/1

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Developmental validation of STRmix™ NGS, a probabilistic genotyping tool for the interpretation of autosomal STRs from forensic profiles generated using NGS

Abstract

Keywords

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