TY - JOUR
T1 - Dietary curcumin supplementation enhances intestinal immunity and gill protection in juvenile greater amberjack (Seriola dumerili)
AU - He, Yuhang
AU - Fu, Zhengyi
AU - Dai, Shiming
AU - Yu, Gang
AU - Ma, Zhenhua
AU - Wang, Xiaomei
PY - 2022/11
Y1 - 2022/11
N2 - A 8-week feeding trial was conducted to determine the effects of dietary supplementation with curcumin on growth, intestinal health, and gills resistance to ammonia stress in juvenile Seriola dumerili. Three isonitrogenous and isolipidic test diets were prepared by supplementing incremental levels of dietary curcumin at 0% (CUR0%, control), 0.01% (CUR0.01%), 0.02% (CUR0.02%), respectively. Fish were fed with experimental diet. Recovery and protection capacity after ammonia challenge assay was adopted to test the effect of curcumin. At the end of the feeding trial, the results showed that dietary supplementation with proper curcumin level had a significant positive effect on fish survival and intestinal histology structure. Meanwhile, dietary supplementation with proper curcumin level can improve intestinal health by increasing immune enzyme activity, up-regulating the expression of anti-inflammatory cytokines, down-regulating the expression of pro-inflammatory cytokines and regulating other immune-related genes. Subsequently, after the ammonia challenge and recovery experiment, the results of antioxidant-related genes and antioxidant enzymes showed that dietary supplementation with proper curcumin level can improve the gill protective and recovery capacity from ammonia stress through increasing antioxidant capacity. These results suggest that dietary supplementation with proper curcumin can promote the growth, intestinal health, gill resistance and recovery to ammonia stress of S. dumerili.
AB - A 8-week feeding trial was conducted to determine the effects of dietary supplementation with curcumin on growth, intestinal health, and gills resistance to ammonia stress in juvenile Seriola dumerili. Three isonitrogenous and isolipidic test diets were prepared by supplementing incremental levels of dietary curcumin at 0% (CUR0%, control), 0.01% (CUR0.01%), 0.02% (CUR0.02%), respectively. Fish were fed with experimental diet. Recovery and protection capacity after ammonia challenge assay was adopted to test the effect of curcumin. At the end of the feeding trial, the results showed that dietary supplementation with proper curcumin level had a significant positive effect on fish survival and intestinal histology structure. Meanwhile, dietary supplementation with proper curcumin level can improve intestinal health by increasing immune enzyme activity, up-regulating the expression of anti-inflammatory cytokines, down-regulating the expression of pro-inflammatory cytokines and regulating other immune-related genes. Subsequently, after the ammonia challenge and recovery experiment, the results of antioxidant-related genes and antioxidant enzymes showed that dietary supplementation with proper curcumin level can improve the gill protective and recovery capacity from ammonia stress through increasing antioxidant capacity. These results suggest that dietary supplementation with proper curcumin can promote the growth, intestinal health, gill resistance and recovery to ammonia stress of S. dumerili.
KW - Ammonia stress
KW - Growth performance
KW - Intestinal histology
KW - Intestinal immune responses
KW - Protective effect
KW - Recovery capacity
UR - http://www.scopus.com/inward/record.url?scp=85146226157&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2022.e11887
DO - 10.1016/j.heliyon.2022.e11887
M3 - Article
AN - SCOPUS:85146226157
SN - 2405-8440
VL - 8
JO - Heliyon
JF - Heliyon
IS - 11
M1 - e11887
ER -
