Different levels of immunoreactivity for synaptosomal-associated protein of 25 kDa in vasoconstrictor and vasodilator axons of guinea-pigs

Judy L. Morris, Catharina E.Y. Lindberg, Ian L. Gibbins

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Immunoreactivity (IR) for synaptosomal-associated protein of 25 kDa (SNAP-25) was examined in axons of autonomic vasoconstrictor and vasodilator neurons innervating the lingual and uterine arteries of guinea-pigs. Polyacrylamide gel electrophoresis and immunoblotting of protein extracts demonstrated a SNAP-25-IR band at 25 kDa in both arteries. Quantitative confocal microscopy demonstrated significantly higher levels of SNAP-25-IR in varicosities with IR for vasoactive intestinal peptide (VIP) than in adjacent axons with IR for tyrosine hydroxylase (TH). Levels of SNAP-25-IR in TH-IR axons, relative to adjacent VIP-IR axons, were significantly higher in the lingual artery than the uterine artery. These differences in IR for SNAP-25, a protein considered essential for calcium-dependent exocytosis of neurotransmitters, raise the possibility that mechanisms of transmitter release may vary between different classes of autonomic neurons. Copyright (C) 2000 Elsevier Science Ireland Ltd.

    Original languageEnglish
    Pages (from-to)167-170
    Number of pages4
    JournalNeuroscience letters
    Volume294
    Issue number3
    DOIs
    Publication statusPublished - 24 Nov 2000

    Keywords

    • Arteries
    • Autonomic neurons
    • Exocytosis
    • Neurotransmission
    • SNARE proteins
    • Synaptic vesicles

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