TY - JOUR
T1 - Differential cellular recognition of antigens during acute Plasmodium falciparum and Plasmodium vivax malaria
AU - Salwati, Ervi
AU - Minigo, Gabriela
AU - Woodberry, Tonia
AU - Piera, Kim
AU - de Silva, Harini
AU - Kenangalem, E
AU - Tjitra, E
AU - Coppel, Ross
AU - Price, Ric
AU - Anstey, Nicholas
AU - Plebanski, Magdalena
PY - 2011/4/15
Y1 - 2011/4/15
N2 - Background. Plasmodium falciparum and Plasmodium vivax are co-endemic in the Asia-Pacific region. Their capacity to induce and sustain diverse T-cell responses underpins protective immunity. We compared T-cell responses to the largely conserved merozoite surface protein-5 (PfMSP5) during acute and convalescent falciparum and vivax malaria. Methods. Lymphoproliferation and IFN-γ secretion to PfMSP5 and purified protein derivate were quantified in adults with falciparum (n = 34), and vivax malaria (n = 12) or asymptomatic residents (n = 10) of Papua, Indonesia. Responses were reassessed 7-28 days following treatment. Results. The frequency of IFN-γ responders to PfMSP5 was similar in acute falciparum (63%) or vivax (67%) malaria. However, significantly more IFN-γ-secreting cells were detectable during vivax compared with falciparum infection. Purified protein derivative responses showed a similarly enhanced pattern. While rapidly lost in vivax patients, PfMSP5-specific responses in falciparum malaria remained to day 28. By contrast, frequency and magnitude of lymphoproliferation to PfMSP5 were similar for falciparum and vivax infections. Conclusion. Cellular PfMSP5-specific responses are most frequent during either acute falciparum or vivax malaria, indicating functional T-cell responses to conserved antigens. Both effector and central memory T-cell functions are increased. Greater IFN-γ responses in acute P. vivax, suggest enhancement of pre-existing effector T-cells during acute vivax infection.
AB - Background. Plasmodium falciparum and Plasmodium vivax are co-endemic in the Asia-Pacific region. Their capacity to induce and sustain diverse T-cell responses underpins protective immunity. We compared T-cell responses to the largely conserved merozoite surface protein-5 (PfMSP5) during acute and convalescent falciparum and vivax malaria. Methods. Lymphoproliferation and IFN-γ secretion to PfMSP5 and purified protein derivate were quantified in adults with falciparum (n = 34), and vivax malaria (n = 12) or asymptomatic residents (n = 10) of Papua, Indonesia. Responses were reassessed 7-28 days following treatment. Results. The frequency of IFN-γ responders to PfMSP5 was similar in acute falciparum (63%) or vivax (67%) malaria. However, significantly more IFN-γ-secreting cells were detectable during vivax compared with falciparum infection. Purified protein derivative responses showed a similarly enhanced pattern. While rapidly lost in vivax patients, PfMSP5-specific responses in falciparum malaria remained to day 28. By contrast, frequency and magnitude of lymphoproliferation to PfMSP5 were similar for falciparum and vivax infections. Conclusion. Cellular PfMSP5-specific responses are most frequent during either acute falciparum or vivax malaria, indicating functional T-cell responses to conserved antigens. Both effector and central memory T-cell functions are increased. Greater IFN-γ responses in acute P. vivax, suggest enhancement of pre-existing effector T-cells during acute vivax infection.
UR - http://www.scopus.com/inward/record.url?scp=79953320405&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiq166
DO - 10.1093/infdis/jiq166
M3 - Article
VL - 203
SP - 1192
EP - 1199
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 8
ER -