TY - JOUR
T1 - Differential distribution of substance P binding sites in guinea-pig sympathetic ganglia
AU - Messenger, J. P.
AU - Gibbins, I. L.
PY - 1998/4/30
Y1 - 1998/4/30
N2 - We have used a combination of autoradiographic and immunohistochemical techniques to investigate the distribution of binding sites for substance P in relation to the distribution of substance P-immunoreactive nerve fibres and specific functional populations of neurons in the sympathetic ganglia of guinea-pigs. There was considerable heterogeneity in the density of binding sites for Bolton Hunter labelled 125I - substance P (BHSP). Binding sites were more dense in the prevertebral ganglia, such as the coeliac and inferior mesenteric ganglia, than in the paravertebral ganglia, such as the superior cervical or lumbar chain ganglia. The binding sites tended to be clumped within the ganglia. Within the prevertebral ganglia, they were associated predominantly with neurons projecting to the enteric plexuses. Many of these neurons contained somatostatin immunoreactivity. In the lumbar sympathetic chain ganglia, there was a weak association of binding sites with neurons containing immunoreactivity to vasoactive intestinal peptide. Overall, the density of binding sites matched the density of nerve fibres containing immunoreactivity to substance P in different ganglia. However, within particular ganglia, there was little, if any, correlation between the distribution of binding sites and nerve fibres containing substance P. Most of the binding sites in the ganglia had the pharmacological characteristics of NK1 receptors. Our results show that there is considerable heterogeneity in the expression of NK1 receptors in the sympathetic ganglia of guinea- pigs. However, given the relatively poor spatial correlation between the distribution of binding sites and potential sites of substance P release from intraganglionic nerve fibres, we suggest that substance P may diffuse for relatively large distances through the ganglia, with actions only on those neurons selectively expressing NK1 receptors.
AB - We have used a combination of autoradiographic and immunohistochemical techniques to investigate the distribution of binding sites for substance P in relation to the distribution of substance P-immunoreactive nerve fibres and specific functional populations of neurons in the sympathetic ganglia of guinea-pigs. There was considerable heterogeneity in the density of binding sites for Bolton Hunter labelled 125I - substance P (BHSP). Binding sites were more dense in the prevertebral ganglia, such as the coeliac and inferior mesenteric ganglia, than in the paravertebral ganglia, such as the superior cervical or lumbar chain ganglia. The binding sites tended to be clumped within the ganglia. Within the prevertebral ganglia, they were associated predominantly with neurons projecting to the enteric plexuses. Many of these neurons contained somatostatin immunoreactivity. In the lumbar sympathetic chain ganglia, there was a weak association of binding sites with neurons containing immunoreactivity to vasoactive intestinal peptide. Overall, the density of binding sites matched the density of nerve fibres containing immunoreactivity to substance P in different ganglia. However, within particular ganglia, there was little, if any, correlation between the distribution of binding sites and nerve fibres containing substance P. Most of the binding sites in the ganglia had the pharmacological characteristics of NK1 receptors. Our results show that there is considerable heterogeneity in the expression of NK1 receptors in the sympathetic ganglia of guinea- pigs. However, given the relatively poor spatial correlation between the distribution of binding sites and potential sites of substance P release from intraganglionic nerve fibres, we suggest that substance P may diffuse for relatively large distances through the ganglia, with actions only on those neurons selectively expressing NK1 receptors.
KW - Autoradiography
KW - Neurokinin receptor
KW - Preganglionic
KW - Sensory
KW - Substance P
KW - Sympathetic ganglia
UR - http://www.scopus.com/inward/record.url?scp=0032580198&partnerID=8YFLogxK
U2 - 10.1016/S0165-1838(98)00007-1
DO - 10.1016/S0165-1838(98)00007-1
M3 - Article
C2 - 9696265
AN - SCOPUS:0032580198
SN - 0165-1838
VL - 69
SP - 103
EP - 114
JO - Journal of the Autonomic Nervous System
JF - Journal of the Autonomic Nervous System
IS - 2-3
ER -